Agilent 6890 GC/5973 mass detector for Profiling

用于分析的 Agilent 6890 GC/5973 质量检测器

• 批准号: 6877331
• 负责人: ROGER A DAVIS
• 金额: $ 11.68万
• 依托单位: SAN DIEGO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-05 至 2006-04-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877331
• 关键词: biomedical equipment purchase; gas chromatography mass spectrometry; lipid metabolism; liquid chromatography mass spectrometry; oxidized lipid

DESCRIPTION (provided by applicant): We are a group of investigators with long-term collaborations centering on our common interest in metabolic disease and atherosclerosis. The Davis lab's contribution to the technical expertise of the group has been to provide lipid quantitation and oxysterol analysis using gas/liquid chromatography and GC/mass spectroscopy. The purpose of this application is to seek funds to procure a GC/mass spectrometer allowing us to analyze cells, tissues and isolates for lipids, sterols and metabolites. In addition, this machine will enable us to perform stable isotope dilution analysis in order to assess how phenotype (i.e. the in vivo metabolism of lipids, sterols and metabolites) may be linked to the genetic background of specific mice. We request an Agilent 6890 GC having a split/splitless all glass injector connected to a flame ionization detector as well as a 5973 mass selective detector enabling us to quantitate cholesterol/sterols/fatty acids and to analyze the metabolic profiles of congenic and genetically altered mice as a means to link genomics with metabolic phenotypes with emphasis on susceptibility to atherosclerosis. This equipment is needed to replace the Davis lab's 19 year old Hewlett-Packard 5890A GC having a split/splitless all glass injector connected to a flame ionization detector as well as a 5970 mass selective detector. During the past 3 years, the Davis lab has spent over $8000 to have this instrument repeatedly fixed using salvaged parts. Unfortunately, this was a waste of time and money, as many parts to the machine were outdated and replacement not possible. We have now have been forced to agree with Agilent that our old machine can not be fixed in a reliable manner. It is for this reason that we seek funds to purchase a replacement GC/mass spectrometer. Each lab has used GC sterol analysis as shown in table 1. Because of our dependence on this technique, prior to breaking down we used the GC -60% of the time. The projected use of each investigator is shown in table 1 and will account for 100% time use. Therefore, we have no alternative equipment for this essential technique.

描述(申请人提供)：我们是一群长期合作的研究人员，围绕着我们对代谢性疾病和动脉粥样硬化的共同兴趣。戴维斯实验室对该小组的技术专长的贡献是使用气/液色谱和GC/MS提供脂类定量和氧固醇分析。这项申请的目的是寻求资金，以采购一台气相色谱/质谱仪，使我们能够分析细胞、组织和分离的类脂、甾醇和代谢物。此外，这台机器将使我们能够进行稳定的同位素稀释分析，以评估表型(即脂类、甾醇和代谢物的体内代谢)如何与特定小鼠的遗传背景有关。我们需要一台Agilent 6890 GC，它有一个连接到火焰电离检测器的分离式/无分离式全玻璃进样器，以及一个5973质量选择性检测器，使我们能够对胆固醇/甾醇/脂肪酸进行定量，并分析同种基因和基因改变小鼠的代谢谱，作为一种将基因组学与代谢表型联系起来的手段，重点是动脉粥样硬化的易感性。戴维斯实验室已有19年历史的惠普5890A GC需要更换该设备，该GC具有一个连接到火焰电离检测器和5970质量选择检测器的分体式/无分体式全玻璃注射器。在过去的三年里，戴维斯实验室花费了超过8000美元来反复使用回收的部件来修复这台仪器。不幸的是，这是浪费时间和金钱，因为机器的许多部件都过时了，无法更换。我们现在不得不同意安捷伦的观点，即我们的旧机器不能以可靠的方式进行修复。正是出于这个原因，我们寻求资金购买一台更换的GC/MS光谱仪。每个实验室都使用了如表1所示的GC甾醇分析。由于我们对这项技术的依赖，在故障发生之前，我们使用GC-60%的时间。每名调查员的预计使用量见表1，将占100%的时间使用量。因此，对于这项关键技术，我们没有替代设备。