Agilent 6890 GC/5973 mass detector for Profiling

用于分析的 Agilent 6890 GC/5973 质量检测器

• 批准号: 6877331
• 负责人: ROGER A DAVIS
• 金额: $ 11.68万
• 依托单位: SAN DIEGO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-05 至 2006-04-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877331
• 关键词: biomedical equipment purchase; gas chromatography mass spectrometry; lipid metabolism; liquid chromatography mass spectrometry; oxidized lipid

DESCRIPTION (provided by applicant): We are a group of investigators with long-term collaborations centering on our common interest in metabolic disease and atherosclerosis. The Davis lab's contribution to the technical expertise of the group has been to provide lipid quantitation and oxysterol analysis using gas/liquid chromatography and GC/mass spectroscopy. The purpose of this application is to seek funds to procure a GC/mass spectrometer allowing us to analyze cells, tissues and isolates for lipids, sterols and metabolites. In addition, this machine will enable us to perform stable isotope dilution analysis in order to assess how phenotype (i.e. the in vivo metabolism of lipids, sterols and metabolites) may be linked to the genetic background of specific mice. We request an Agilent 6890 GC having a split/splitless all glass injector connected to a flame ionization detector as well as a 5973 mass selective detector enabling us to quantitate cholesterol/sterols/fatty acids and to analyze the metabolic profiles of congenic and genetically altered mice as a means to link genomics with metabolic phenotypes with emphasis on susceptibility to atherosclerosis. This equipment is needed to replace the Davis lab's 19 year old Hewlett-Packard 5890A GC having a split/splitless all glass injector connected to a flame ionization detector as well as a 5970 mass selective detector. During the past 3 years, the Davis lab has spent over $8000 to have this instrument repeatedly fixed using salvaged parts. Unfortunately, this was a waste of time and money, as many parts to the machine were outdated and replacement not possible. We have now have been forced to agree with Agilent that our old machine can not be fixed in a reliable manner. It is for this reason that we seek funds to purchase a replacement GC/mass spectrometer. Each lab has used GC sterol analysis as shown in table 1. Because of our dependence on this technique, prior to breaking down we used the GC -60% of the time. The projected use of each investigator is shown in table 1 and will account for 100% time use. Therefore, we have no alternative equipment for this essential technique.

描述（由申请人提供）：我们是一组长期合作的研究人员，专注于我们对代谢疾病和动脉粥样硬化的共同兴趣。戴维斯实验室对该小组技术专长的贡献是使用气相/液相色谱法和GC/质谱法提供脂质定量和氧固醇分析。本申请的目的是寻求资金，以采购GC/质谱仪，使我们能够分析细胞，组织和分离的脂质，甾醇和代谢物。此外，这台机器将使我们能够进行稳定同位素稀释分析，以评估表型（即脂质，固醇和代谢物的体内代谢）如何与特定小鼠的遗传背景相关联。我们要求Agilent 6890 GC具有连接到火焰离子化检测器的分裂/不分裂全玻璃进样器以及5973质量选择性检测器，使我们能够定量胆固醇/甾醇/脂肪酸并分析同类和遗传改变小鼠的代谢谱，作为将基因组学与代谢表型联系起来的手段，重点是动脉粥样硬化的易感性。需要该设备来替换Davis实验室使用了19年的Hewlett-Packard 5890 A GC，该GC具有连接到火焰离子化检测器以及5970质量选择检测器的分裂/不分裂全玻璃进样器。在过去的3年里，戴维斯实验室已经花费了8000多美元，使用回收的部件反复修复该仪器。不幸的是，这是浪费时间和金钱，因为机器的许多部件已经过时，无法更换。我们现在不得不同意安捷伦，我们的旧机器不能以可靠的方式修复。正是出于这个原因，我们寻求资金购买更换GC/质谱仪。每个实验室都使用了GC甾醇分析，如表1所示。由于我们对这种技术的依赖，在分解之前，我们使用GC约60%的时间。每名研究者的预计使用情况见表1，将占100%的时间使用。因此，对于这项基本技术，我们没有替代设备。