Changes in gene expression and alternative polyadenylation in response to Hypoxia

缺氧时基因表达和替代多聚腺苷酸化的变化

• 批准号: 1757819
• 金额: --
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1757819
• 关键词: Changes; gene; expression; alternative; polyadenylation

This project will investigate the role of alternative polyadenylation (APA) in the transcriptomic response to cellular stress. APA is the use of alternative polyadenylation sites during transcription to give mRNA transcripts with varying 3'UTRs. These 3'UTRs often contain signal sequences which influence the transcripts localisation, function and stability. Therefore, APA can greatly affect the transcriptome in response to a specific cellular state. HEK293T cells will be subjected to a range of environmental insults such as reactive oxygen species and Ultraviolet radiation. Nuclear and cytoplasmic mRNA will be isolated from the cells and sequenced using RNA-Seq. Bioformatic analysis will evaluate any changes in the APA profile and identify key alterations to the transcriptome which will then undergo further investigation.BBSRC Priority Areas:Systems approaches to the biosciencesData driven biologyWUB, ENWW

本项目将研究交替多聚腺苷酸化（阿帕）在转录组对细胞应激反应中的作用。阿帕是在转录过程中使用替代的多聚腺苷酸化位点，以产生具有不同3 'UTR的mRNA转录物。这些3 'UTR通常含有影响转录本定位、功能和稳定性的信号序列。因此，阿帕可以极大地影响转录组以响应特定的细胞状态。HEK 293 T细胞将经受一系列环境损伤，例如活性氧和紫外线辐射。将从细胞中分离细胞核和细胞质mRNA，并使用RNA-Seq进行测序。生物信息学分析将评估阿帕谱中的任何变化，并确定转录组的关键改变，然后进行进一步的研究。BBSRC优先领域：生物科学的系统方法数据驱动生物学WUB，ENWW