CORE--LIGAND PREPARATION AND ASSAY

核心——配体制备与检测

• 批准号: 6440530
• 负责人: JOHANNES D VELDHUIS
• 金额: $ 17.42万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440530
• 关键词: biological products; biomedical facility; immunocytochemistry; immunologic assay /test; immunological substance; ligands; method development; radiotracer; reagent /indicator; reproduction; training

The Ligand Assay and Analysis Core will serve virtually all eligible projects identified in this U54 Center application. In brief, the Core will: (1) prepare and purify labeled ligands for assays, agonist/antagonist binding studies, or immunocytochemistry; (2) provide estimates of the concentration of hormones or other regulatory molecules by RIA, chemiluminescent, immunometric, and bioassay procedures; (3) transfer relevant ligand preparation and assay technology procedures to projects; and (4) develop (10% effort) new or improved procedures required by the evolving investigative needs of funded projects eligible for Core access. The primary drive for establishing this Core laboratory is to provide high-quality technical services to U54 Center-related projects that require assay reagents, labeled ligands, and estimates of hormone concentrations. This services will benefit multiple U54 and Reproductive Sciences projects, since no mechanisms are available otherwise to obtain high-volume assays and/or high purified ligands required for investigations with isolated cells or particulate fractions of reproductive cells or tissue. Projects requiring new ligands or assays cannot justify the time or effort of a technician to establish, validate, and perform these procedures. A central facility will allow the U54 Center to meet the needs of all projects, enhance their productivity, implement a uniform set rationale for proposing this Core is to provide an efficient mechanism whereby new or improved (including non-radioactive) methods can be implemented for estimating amounts of peptides, growth factors, and steroids in multiple cell-culture and body fluids. This Ligand Assay and Analysis Core will thus serve the immediate and future needs of eligible reproductive-science projects in this U54 Center that require a dedicated ligand and assay central facility to conduct, develop, validate, and implement relevant measurement and analysis techniques.

配体检测和分析核心将服务于几乎所有符合条件的 在U 54中心申请中确定的项目。简言之，核心 将：（1）制备和纯化用于测定的标记配体， 激动剂/拮抗剂结合研究或免疫细胞化学;（2）提供 激素或其他调节分子浓度的估计 通过放射免疫分析、荧光分析、免疫测定和生物测定程序;（3） 将相关配体制备和测定技术程序转移至 （4）开发（10%的努力）所需的新程序或改进程序 符合核心预算的受资助项目不断变化的调查需求 access. 建立该核心实验室的主要驱动力是提供 为U 54中心相关项目提供高质量的技术服务， 需要测定试剂、标记配体和激素的估计， 浓度的这项服务将有利于多个U 54和生殖 科学项目，因为没有其他机制可以获得 高容量测定和/或高纯度配体 分离的细胞或颗粒部分的研究 生殖细胞或组织。需要新配体或检测试剂的项目 不能证明技术人员建立、验证 并执行这些程序。中央设施将允许U 54中心 以满足所有项目的需要，提高其生产力，实施 提出这一核心的一个统一的基本原理是提供一个有效的 新的或改进的（包括非放射性）方法可以 用于估计肽、生长因子和 多种细胞培养和体液中的类固醇该配体测定和 因此，分析核心将满足符合条件的人的当前和未来需求。 生殖科学项目在这个U 54中心，需要一个专门的 配体和测定中心设施进行，开发，验证， 实施相关测量和分析技术。