MULTIDISCIPLINARY PROGRAM IN FEMALE REPRODUCTION

年专业合作中心计划的目标 马里兰医学大学生殖研究(SCCPRR) 该中心致力于创新的基础和临床研究 生殖科学并应用从这些科学中获得的知识 提高对本病病因和治疗的认识的研究 女性不孕不育。多学科的实验方法将 在这个项目中被用来研究垂体-卵巢的功能- 子宫轴与细胞重塑这一中心主题有关。 在项目I(Karen A.Gregerson，PI)中， 一种内向整流的多巴胺激活的垂体钾通道 转基因小鼠的催乳素分泌和生育力将被确定 其中该新型膜通道的功能通过以下方式中断 显性-负性突变。在项目II(杰拉尔德·J·佩佩，派)中， 雌激素对胎儿卵泡和卵母细胞的调节作用 将在#年对狒狒的发育和对生育的影响进行评估。 胎盘雌激素合成在子宫内受抑制的是什么？ 给药一种新的芳香酶抑制剂。在项目III中(罗伯特 D.Koos，Pi)，成纤维细胞生长成员的生理作用 碱性成纤维细胞生长因子和角质形成细胞生长因子家族在卵巢和卵巢中的作用 将在#年用转基因小鼠研究子宫功能和生育能力。 截短的显性阴性的成纤维细胞生长因子受体在空间上和 暂时过度表达。一项临床和基础研究相结合的研究 在项目四(尤金·D·阿尔布雷希特，PI)中提出，其中 雌激素和孕激素，通过血管内皮细胞生长起作用 通透性因子和碱性成纤维细胞生长因子对子宫内膜血管生成和生育力的影响 将在狒狒体内和体外在人类身上进行研究 子宫内膜。每一项研究项目都将大量借鉴 细胞免疫细胞化学/原位杂交核心(Gloria E.Hoffman， PI)用于调节肽和类固醇的细胞定位 荷尔蒙受体。将科学工作整合到这项《SCCPRR》 将通过聚焦于细胞重塑的主题来实现， 共同的技术分子方法和既定的传统 合作研究的成果。

The objective of the Specialized Cooperative Centers Program in Reproduction Research (SCCPRR) at the University of Maryland Medical Center is to conduct innovative basic and clinical research in the reproductive sciences and to apply the knowledge gained from these studies to improve understanding of the etiology and treatment of infertility in women. A multidisciplinary experimental approach will be taken in this program to study function of the pituitary-ovarian- uterine axis as related to the central theme on cellular remodeling. In Project I (Karen A. Gregerson, PI), the physiological importance of an inwardly-rectifying, dopamine-activated pituitary K+ channel to prolactin secretion and fertility will be determined in transgenic mice in which the function of this novel membrane channel is interrupted by dominant-negative mutation. In Project II (Gerald J. Pepe, PI), the regulatory role of estrogen on fetal ovarian follicular and oocyte development and the impact on fertility will be assessed in baboons in which placental estrogen synthesis is suppressed in utero by administration of a novel aromatase inhibitor. In Project III (Robert D. Koos, PI), the physiological role of members of the fibroblast growth factor family, bFGF and keratinocyte growth factor, on ovarian and uterine function and fertility will be studied in transgenic mice in which the truncated, dominant-negative FGF receptors are spatially and temporally overexpressed. A combined clinical-basic research study is proposed in Project IV (Eugene D. Albrecht, PI), in which the roles of estrogen and progesterone, acting via vascular endothelial growth/ permeability factor and bFGF, on endometrial angiogenesis and fertility will be investigated in vivo in baboons and in vitro in the human endometrium. Each of the research projects will draw heavily upon the Cell Immunocytochemistry/In Situ Hybridization Core (Gloria E. Hoffman, PI) for the cellular localization of regulatory peptides and steroid hormone receptors. Integration of the scientific effort in this SCCPRR will be achieved through the thematic focus on cellular remodeling, common technological molecular approaches, and an established tradition of collaborative research.