CONFOCAL SCANNING LASER MICROSCOPE

共焦扫描激光显微镜

• 批准号: 6052108
• 负责人: Nae J Dun
• 金额: $ 26万
• 依托单位: EAST TENNESSEE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6052108
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy

This request is for a Bio-Rad Confocal Scanning Laser Microscope system in conjunction with a Zeiss microscope equipped with episcopic fluorescence optics. The system will significantly enhance the research programs of four major users who are conducting N.I.H. funded R01 research projects and three users, two of whom are R15 awardees. The microscope system will be employed in several distinctly different ways. Drs. Dun, Ardell and Hoover will employ the microscope system to reconstruct brainstem, spinal neurons and intracardiac ganglion cells filled with lucifer yellow or biotin-dextran in vitro preparations and to image the spatial relationship between nerve terminals containing a specific neurotransmitter and dye-filled neuron. Dr. Ganote will determine the site(s) of translocation of heat shock proteins and several other cytoskeletal proteins in myocytes. Dr. Defore will examine the role of integrin adhesion receptors in attachment-dependent survival of retinal pigment epithelial cells in vitro, using confocal microscopy to image both ligand-activated and clustered receptors. Dr. Harrison will examine gustatory axon terminals in relation to hypothalamic neurons containing specific neuropeptides that influence food intake and feeding behavior. Dr. Ecay will address the issue of trafficking of myosin-coated trans- Golgi network vesicles to apical and/or basolateral membrane domains. The Bio-Rad system is the most optimal instrument available for the applications outlined here. The investigators whose projects comprise this application have evaluated the systems developed by Bio-Rad, Zeiss and Olympus. The preliminary data included in the application were obtained using the Bio-Rad microscope. The East Tennessee State University College of Medicine has a strong institutional commitment to the acquisition and maintenance of the microscope system. A highly experienced and full-time research assistant, Mr. David Neely, will oversee the day-to-day operation of the microscope and a room about 100 ft/2 in the Department of Pharmacology will be renovated to house the system. The College of Medicine has also agreed to underwrite the service contract for the instrument, if it is purchased.

该请求是针对Bio-Rad共焦扫描激光显微镜系统与配备episcopic荧光光学器件的Zeiss显微镜的结合。该系统将大大增强进行N.I.H.的四个主要用户的研究计划。资助的R 01研究项目和三个用户，其中两个是R15获奖者。显微镜系统将以几种明显不同的方式使用。Dun，Ardell和Hoover博士将使用显微镜系统重建脑干，脊髓神经元和充满荧光黄或生物素-葡聚糖的心内神经节细胞体外制剂，并对含有特定神经递质的神经末梢和充满染料的神经元之间的空间关系进行成像。Ganote博士将确定热休克蛋白和肌细胞中其他几种细胞骨架蛋白的易位位点。Defore博士将研究整合素粘附受体在体外视网膜色素上皮细胞粘附依赖性存活中的作用，使用共聚焦显微镜对配体激活和聚集的受体进行成像。哈里森博士将研究味觉轴突终端与下丘脑神经元的关系，下丘脑神经元含有影响食物摄入和喂养行为的特定神经肽。Ecay博士将讨论肌球蛋白包被的反式高尔基体网络囊泡向顶端和/或基底外侧膜结构域的运输问题。Bio-Rad系统是适用于本文所述应用的最佳仪器。研究人员的项目包括这个应用程序已经评估了系统开发的Bio-Rad，蔡司和奥林巴斯。申请中包含的初步数据是使用Bio-Rad显微镜获得的。东田纳西州立大学医学院对显微镜系统的获取和维护有很强的机构承诺。一位经验丰富的全职研究助理大卫尼利先生将监督显微镜的日常操作，药理学系的一个大约100英尺/2的房间将被翻新以容纳该系统。医学院还同意承保仪器的服务合同，如果它被购买。