ACTIVITY OF BRAINSTEM NEURONS

脑干神经元的活动

• 批准号: 2415613
• 负责人: Nae J Dun
• 金额: $ 14.71万
• 依托单位: EAST TENNESSEE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-05-01 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2415613
• 关键词: action potentials; brain electrical activity; gamma aminobutyrate; glutamate receptor; glutaminase; glycine; immunocytochemistry; laboratory rat; magnesium; medulla oblongata; neural inhibition; neural transmission; neuroanatomy; neuronal transport; neuropharmacology; potassium channel; synapses; voltage /patch clamp; voltage gated channel

It has long been recognized that a group of neurons in the rostral ventrolateral medulla (RVLM) are an essential component of the central network for maintaining sympathetic vasomotor tone and respiration. Chemical or electrolytic lesions in the RVLM cause a rapid and sustained fall in blood pressure and disruption of respiration in experimental animals. Similar changes in cardio-respiratory dynamics are noted in humans suffering from high cervical cord trauma. To study in depth the electrical behavior and synaptic mechanism of RVLM neurons, an area virtually unexplored, an experimental model consisting of a brainstem- spinal cord preparation isolated from young (25-30 days) rats has now been developed. New preliminary data from our model has shown that a group of neurons in the RVLM are intrinsically active. Whole-cell patch clamp recordings will be made from bulbospinal RVLM neurons identified antidromically by stimulation of the lateral funiculus at the T2 segment or by the presence of the retrograde tracer fluorogold injected into the white matter dorsolateral to the intermediolateral cell column of T2 and T3 segments 3 days prior to experimentation. The following specific aims will be addressed. First, do spinally projecting RVLM neurons exhibit different membrane property and synaptic mechanism as do non-spinally projecting RVLM neurons? Second, do the intrinsically active RVLM neurons possess different membrane currents as do the non-spontaneously active neurons? Third, are the intrinsically active RVLM neurons different from the non-active neurons insofar as their transmitter phenotype is concerned? Lastly, do these two types of RVLM neurons receive different synaptic inputs and/or exhibit different synaptic potentials? A comprehensive study of the electrical behavior and synaptic mechanism that distinguish the activity of intrinsically active from quiescent RVLM neurons constitutes a necessary step toward improving our current knowledge of the neural network which is critical for homeostatic control of blood pressure and respiration. Likewise, their dysfunction may contribute to high blood pressure and other cardio-pulmonary disease states.

人们很早就认识到，嘴侧的一组神经元 延髓腹外侧 (RVLM) 是中枢神经系统的重要组成部分 维持交感血管舒缩张力和呼吸的网络。 RVLM 中的化学或电解损伤会导致快速且持续的 实验中血压下降和呼吸中断 动物。 心肺动力学的类似变化见于 患有高位颈髓损伤的人。 为深入研究 RVLM 神经元的电行为和突触机制，一个领域 实际上尚未探索过，一个由脑干组成的实验模型 从幼年（25-30 天）大鼠中分离出的脊髓制剂现已被 发达。 我们模型的新初步数据表明，一组 RVLM 中的神经元本质上是活跃的。 全细胞膜片钳 将从已识别的球脊髓 RVLM 神经元中进行记录 通过刺激 T2 段的侧索而实现逆向性 或通过注入逆行示踪剂氟金的存在 T2 和 T2 中间外侧细胞柱的背外侧白质 实验前 3 天的 T3 片段。 具体目标如下 将得到解决。 首先，脊髓投射的 RVLM 神经元是否表现出 与非脊髓不同的膜特性和突触机制 投射 RVLM 神经元？ 其次，做本质上活跃的RVLM神经元 与非自发活性物质一样具有不同的膜电流 神经元？ 第三，本质上活跃的 RVLM 神经元与 非活跃神经元的递质表型是 担心的？最后，这两种类型的 RVLM 神经元接收到的信号是否不同？ 突触输入和/或表现出不同的突触电位？一个 电行为和突触机制的综合研究 区分本质活跃的 RVLM 和静态 RVLM 的活动 神经元构成了改善我们当前状况的必要步骤 对稳态控制至关重要的神经网络知识 血压和呼吸。 同样，他们的功能障碍可能 导致高血压和其他心肺疾病 州。