ACTIVITY OF BRAINSTEM NEURONS
脑干神经元的活动
基本信息
- 批准号:6389317
- 负责人:
- 金额:$ 22.22万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1995
- 资助国家:美国
- 起止时间:1995-05-01 至 2004-06-30
- 项目状态:已结题
- 来源:
- 关键词:action potentials brain electrical activity brain mapping brain stem cardiovascular function central neural pathway /tract fos protein hormone regulation /control mechanism hypothalamic hormones hypothalamus immunocytochemistry immunologic assay /test laboratory rat medulla oblongata microinjections neural inhibition neural transmission neuropeptides neuroregulation potassium channel protein localization voltage /patch clamp voltage gated channel
项目摘要
DESCRIPTION (Verbatim from Applicant's abstract): Epidemiological data show
that obesity is associated with an increased risk of hypertension. The
pathophysiological processes underlying this frequent association are poorly
understood. Orexin A and B are two recently isolated peptides expressed in a
population of neurons in the rat lateral hypothalamus. The peptide upon
intraventricular injection increases food consumption in rodents. Orexin
A-immunoreactive (ORXA-LI) fibers project to areas of the rat brainstem
including the rostral ventrolateral medulla (RVLM), the nucleus of the solitary
tract, nucleus ambiguous and the dorsal motor nucleus of the vagus that provide
direct control of autonomic neurons. The presence of ORXA-LI fibers in the RVLM
raises the possibility that the peptide may also be involved in the medullary
regulation of the sympathetic outflow to the spinal cord. Our initial study
shows that orexin A and B injected intracisternally or microinjected into the
RVLM increased blood pressure and heart rate in anesthetized rats. In this
renewal application, a multidisciplinary approach will be employed to test the
hypothesis that orexin A and B may interact with RVLM neurons and enhance
sympathetic outflow in the rat. First the distribution of orexin-like
immunoreactivity in the rat brain will be characterized. Second the effects of
orexin A or B by microinjection to the RVLM area on blood pressure and heart
rate in awake rats will be assessed. Third, whole cell patch recordings will be
made from RVLM neurons of brainstem-spinal cord preparations or coronal
brainstem slices. Recorded neurons will be labeled with Lucifer yellow and
later processed for PNMT-immunoreactivity for identification purposes. The
effects of orexin A or B on the electrical activity of RVLM neurons will be
studied with respect to ionic and signal transduction mechanism. Overweight and
hypertension are two major health problems in the United States. Identification
of the putative transmitters and the circuitry linking the hypothalamus to the
autonomic nervous system will be a significant improvement in our understanding
of the regulatory mechanisms underlying these disorders.
描述(来自申请人摘要的逐字描述):流行病学数据显示
肥胖与高血压风险增加有关。的
这种频繁关联背后的病理生理过程
明白增食欲素A和B是两种最近分离的肽,它们表达在一种表达载体中。
大鼠外侧下丘脑的神经元群体。肽在
脑室内注射增加啮齿动物的食物消耗。食欲素
A-免疫反应(ORXA-LI)纤维投射到大鼠脑干的区域
包括延髓头端腹外侧区(RVLM),孤束核,
迷走神经束、疑核和背侧运动核,
自主神经元的直接控制。RVLM中存在ORXA-LI纤维
提出了这种肽也可能参与骨髓
调节交感神经流出到脊髓。我们最初的研究
显示了脑池内注射或微注射到脑内的食欲素A和B,
RVLM增加麻醉大鼠的血压和心率。在这
续期申请,将采用多学科方法来测试
假设食欲素A和B可能与RVLM神经元相互作用,
大鼠交感神经流出。首先是食欲素样蛋白的分布
将表征大鼠脑中的免疫反应性。第二,影响
通过向RVLM区域微量注射食欲素A或B对血压和心脏的影响
将评估清醒大鼠中的速率。第三,整个细胞补丁记录将是
由脑干-脊髓制备物或冠状面的RVLM神经元制成
脑干切片记录的神经元将用荧光黄标记,
随后进行PNMT免疫反应性处理以用于鉴定目的。的
食欲素A或B对RVLM神经元电活动的影响将被
研究了离子和信号转导机制。超重和
高血压是美国的两大健康问题。识别
下丘脑和大脑皮层之间的联系
自主神经系统将是我们理解的一个重大进步
这些疾病背后的调节机制。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('Nae J Dun', 18)}}的其他基金
NEUROBIOLOGY OF ENDOMORPHINS IN SPINAL DORSAL HORN
脊髓背角内啡肽的神经生物学
- 批准号:
6052393 - 财政年份:1999
- 资助金额:
$ 22.22万 - 项目类别:
NEUROBIOLOGY OF ENDOMORPHINS IN SPINAL DORSAL HORN
脊髓背角内啡肽的神经生物学
- 批准号:
6330625 - 财政年份:1999
- 资助金额:
$ 22.22万 - 项目类别:
NEUROBIOLOGY OF ENDOMORPHINS IN SPINAL DORSAL HORN
脊髓背角内啡肽的神经生物学
- 批准号:
6477173 - 财政年份:1999
- 资助金额:
$ 22.22万 - 项目类别:
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