VARIAN ASSOCIATES UNITY INOVA NMR SPECTROMETER CONSOLE

本申请的目的是使用Varian Associates UNITY INOVA升级生理化学NMR实验室的GE Omega NMR光谱仪。具体而言，升级将取代现有系统的所有元素（控制台，探头，室温恒温器等）。除了磁铁。然而，磁铁将被翻新（断电，更换密封件，重新通电，磁场映射和超导填隙）。布里格姆妇女医院医学部心脏病学分部的核磁共振生理化学实验室目前支持10个NIH资助的项目。这些国家卫生研究院的项目是基于一些机构：布里格姆妇女医院，贝丝以色列女执事医疗中心，和波士顿大学医学院。由NMR生理化学实验室支持的NIH项目主要研究离体灌注的小鼠和大鼠心脏。研究的其他生物系统包括完整的大鼠和小鼠腿、分离的细胞和提取物样本。GE Omega NMR光谱仪现已使用十年，升级的理由是为所支持的项目提供重要的新功能，并为用户群体提供更可靠和可用的资源。当前NIH资助项目的科学目标要求系统性能达到当前系统无法满足的水平。这在离体灌注小鼠心脏的研究中尤其明显，其中31P NMR实验中可用的信噪比和时间分辨率是不够的。Varian UNITY INOVA控制台将大大提高信噪比性能。Varian系统的当前噪声系数比GE Omega新系统的噪声系数低至少2 dB。此外，十年的艰苦使用已经降低了许多关键组件的性能，特别是探头。更换这些旧探头也会增加信噪比。第二个问题是GE欧米茄的可靠性。GE Omega系统的许多关键组件已不再可用。当发生故障时，必须将部件送去修理或翻新。这导致无法使用仪器进行所有研究的时间越来越长。对仪器时间的巨大需求和许多项目研究的时间关键性（例如，特定发育阶段的转基因动物和肥大的外生动物模型）使得随机和长时间的不可用变得非常昂贵和具有破坏性。 瓦里安UNITY INOVA将大大提高可靠性和持续、可预测的可用性。

The objective of this application is to upgrade the GE Omega NMR spectrometer of the NMR Laboratory for Physiological Chemistry with a Varian Associates UNITY INOVA. Specifically, the upgrade will replace all elements of the existing system (console, probes, room temperature shims, etc.) except for the magnet. The magnet will, however, be refurbished (de-energized, seals replaced, re-energized, field mapped, and supercon shimmed). The NMR Laboratory for Physiological Chemistry in the Cardiology Division of the Department of Medicine at Brigham and Women's Hospital currently supports ten NIH funded projects. These NIH projects are based at a number of institutions: the Brigham and Women's Hospital, the Beth Israel Deaconess Medical Center, and the Boston University School of Medicine. The NIH projects supported by the NMR Laboratory for Physiological Chemistry are primarily studying the isolated perfused mouse and rat heart. Other biological systems studied include intact rat and mouse leg, isolated cells, and extract samples. The rationale for upgrading the GE Omega NMR spectrometer, which is now ten years old, is to provide significant new capabilities to the supported projects and to provide a more reliable and available resource for the user community. The scientific aims of the current NIH funded projects demand a level of system performance that can not be met by the current system. This is especially evident in the studies of the isolated perfused mouse heart where the signal to noise and time resolution available in 31P NMR experiments is not adequate. The Varian UNITY INOVA console will have much improved signal to noise performance. Current noise figures for Varian system are at least 2 dB below those of GE Omega when it was new. In addition, ten years of hard use have degraded the performance of many critical components, in particular, the probes. Replacement of these old probes will also increase signal to noise. A second concern is the reliability of the GE Omega. Many critical components of the GE Omega system are no longer available. When breakdowns occur, parts must be sent out for repair or refurbishment. This is resulting in increasingly long periods of time when the instrument is unavailable for all studies. The great demand for instrument time and the time critical nature of many project studies (e.g., transgenic animals of a specific stage of development and exoerimenta1 models of hypertrophy) makes random and lengthy periods of unavailability very expensive and disruptive. The Varian UNITY INOVA will have vastly improved reliability and constant, predictable availability.