ABI PRISM 377 DNA SEQUENCER

本提案要求资助由华盛顿大学分子药理学设施操作的最先进的自动化ABI 377 DNA测序仪。该设施自1989年以来一直在运行，目前我们正以最大容量运行第一代ABI DNA 370A测序仪。我们现在已经到了一个关键阶段，必须升级我们的测序仪器，以提高通量，并在新仪器上提供更高的灵敏度和更长的运行长度。已经开发出了灵敏度更高的染料，我们基于滤光片的激光器无法检测到这些染料，我们预计我们目前的ABI仪器将在明年变得越来越过时。药理学设施是第一个购买，通过美国国立卫生研究院仪器拨款，荧光染料为基础的测序系统，并提供测序在我们部门内外的成本基础上。在过去的10年里，自动化DNA测序技术发展迅速，现在已经在很大程度上取代了整个媒体中心的人工测序。在过去的一年中，来自7个部门的16名主要研究人员占了我们DNA测序请求的75%，他们组成了核心小组，新的仪器将极大地帮助他们继续研究生产力。去年分子药理学研究所（Molecular Pharmacology Facility）剩余的25%的E测序成果已被广泛分配给华盛顿大学26个院系的另外54名研究人员。本提案的资金为NIH资助的一大批高产实验室提供必要和成本效益高的技术服务。

This proposal requests funding for a state of the art automated ABI 377 DNA sequencer to be operated by the Molecular Pharmacology Facility at the University of Washington. This facility has been in operation since 1989 and we are currently operating a first generation ABI DNA 370A sequencer at maximum capacity. We have now reached a critical stage where it is essential to upgrade our sequencing instrumentation in order to increase throughput, and provide the increased sensitivity and longer run lengths possible on the newer instrument. Increased sensitivity dyes have been developed which our filter based laser is unable to detect and we expect our current ABI instrument to become increasingly obsolete over the next year. The Pharmacology Facility was the first to purchase, through an NIH instrumentation grant, a fluorescent dye-based sequencing system and offer sequencing for u both within and outside our Department on a cost basis. Automated DNA sequencing has advanced rapidly over the past 10 years and has now largely replaced manual sequencing throughout the Media Center. A group of 16 principal investigators in 7 departments have accounted for about 75% of our DNA sequencing requests over the past year and they form the core group whose continued research productivity will be greatly assisted by the new instrumentation. The remaining 25% of last year's E sequencing in the Molecular Pharmacology Facility has been broadly distributed among another 54 investigators in 26 departments throughout the University of Washington. Funding of this proposal provide essential and cost effective technical services to a large group of highly productive laboratories funded by the NIH.