ABI PRISM 377 DNA SEQUENCER

这项提案要求为由华盛顿大学分子药理学设施运营的最先进的自动化ABI377 DNA测序仪提供资金。这一设施自以来一直在运营，目前我们正在以最大限度地运行第一代ABI DNA 370A测序仪。我们现在已经到了一个关键阶段，必须升级我们的测序仪器，以增加产量，并提供较新仪器可能的更高灵敏度和更长的运行长度。我们已经开发了灵敏度更高的染料，而我们基于滤光片的激光无法检测到这些染料，我们预计我们目前的ABI仪器将在明年变得越来越过时。药理学机构是第一个通过NIH仪器赠款购买荧光染料测序系统的机构，并以成本为基础为您在我们部门内外提供测序服务。自动DNA测序在过去10年中发展迅速，现在已在很大程度上取代了整个媒体中心的人工测序。在过去的一年里，一个由7个部门的16名首席研究人员组成的小组约占我们DNA测序请求的75%，他们构成了核心小组，其持续的研究生产率将极大地得益于新仪器。去年在分子药理学机构进行的E测序的其余25%已广泛分布在华盛顿大学26个系的另外54名研究人员中。这项提案的资金为由美国国立卫生研究院资助的一大批高生产率实验室提供了基本的和具有成本效益的技术服务。

This proposal requests funding for a state of the art automated ABI 377 DNA sequencer to be operated by the Molecular Pharmacology Facility at the University of Washington. This facility has been in operation since 1989 and we are currently operating a first generation ABI DNA 370A sequencer at maximum capacity. We have now reached a critical stage where it is essential to upgrade our sequencing instrumentation in order to increase throughput, and provide the increased sensitivity and longer run lengths possible on the newer instrument. Increased sensitivity dyes have been developed which our filter based laser is unable to detect and we expect our current ABI instrument to become increasingly obsolete over the next year. The Pharmacology Facility was the first to purchase, through an NIH instrumentation grant, a fluorescent dye-based sequencing system and offer sequencing for u both within and outside our Department on a cost basis. Automated DNA sequencing has advanced rapidly over the past 10 years and has now largely replaced manual sequencing throughout the Media Center. A group of 16 principal investigators in 7 departments have accounted for about 75% of our DNA sequencing requests over the past year and they form the core group whose continued research productivity will be greatly assisted by the new instrumentation. The remaining 25% of last year's E sequencing in the Molecular Pharmacology Facility has been broadly distributed among another 54 investigators in 26 departments throughout the University of Washington. Funding of this proposal provide essential and cost effective technical services to a large group of highly productive laboratories funded by the NIH.