HYDROGEN EXCHANGE STUDIES ON A BETA AMYLOID FIBRILS

描述这项研究提案代表了理解蛋白质聚集相关疾病的整体努力的重要组成部分，包括阿尔茨海默病，亨廷顿病和帕金森病。在患有这些疾病的患者中已经发现蛋白质聚集体和正常可溶性蛋白质的异常组织沉积。重要的是要了解这些聚集体的结构和功能，以便设计诊断和治疗这些疾病的方法。这项提案涉及研究淀粉样纤维的结构，淀粉样纤维是一种聚集体，与阿尔茨海默病有关。分析技术，包括氢氘交换质谱法和微量分离技术将被用来确定氢键和β-折叠网络在淀粉样蛋白原纤维在阿尔茨海默氏病中存在的程度。

DESCRIPTION This research proposal represents an important part of the overall effort to understand protein aggregation associated diseases, including Alzheimer's, Huntington's and Parkinson's Diseases. Protein aggregates and abnormal tissue deposits of normally soluble proteins have been found in the patients suffering from these disease. It is important to understand the structure and function of these aggregates in order to devise methods for diagnosis and cures for these diseases. This proposal addresses studying the structure of amyloid fibrils, a type of aggregate, which is associated with Alzheimer's Disease. Analytical techniques including hydrogen deuterium exchange mass spectrometry and microscale separation techniques will be utilized to define the hydrogen bonding and extent of beta-sheet network in the amyloid fibrils present in Alzheimer's Disease.