HYDROGEN EXCHANGE STUDIES ON A BETA AMYLOID FIBRILS

β 淀粉样原纤维的氢交换研究

• 批准号: 6401987
• 负责人: Indu Kheterpal
• 金额: $ 3.17万
• 依托单位: UNIVERSITY OF TENNESSEE KNOXVILLE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-12-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6401987
• 关键词: Alzheimer's disease; amyloid proteins; chemical models; deuterium; hydrogen bond; mass spectrometry; molecular assembly /self assembly; monomer; neuritic plaques; neurofilament; protein sequence; protein structure function

DESCRIPTION This research proposal represents an important part of the overall effort to understand protein aggregation associated diseases, including Alzheimer's, Huntington's and Parkinson's Diseases. Protein aggregates and abnormal tissue deposits of normally soluble proteins have been found in the patients suffering from these disease. It is important to understand the structure and function of these aggregates in order to devise methods for diagnosis and cures for these diseases. This proposal addresses studying the structure of amyloid fibrils, a type of aggregate, which is associated with Alzheimer's Disease. Analytical techniques including hydrogen deuterium exchange mass spectrometry and microscale separation techniques will be utilized to define the hydrogen bonding and extent of beta-sheet network in the amyloid fibrils present in Alzheimer's Disease.

这项研究计划代表了了解蛋白质聚集相关疾病，包括阿尔茨海默氏症、亨廷顿氏症和帕金森氏症的整体努力的重要部分。在患有这些疾病的患者中发现了蛋白质聚集物和正常可溶性蛋白质的异常组织沉积。重要的是要了解这些聚集体的结构和功能，以便制定诊断和治疗这些疾病的方法。这项建议旨在研究淀粉样纤维的结构，这是一种与阿尔茨海默病相关的聚集体。分析技术包括氢氢交换质谱仪和微型分离技术将被用来确定阿尔茨海默病患者淀粉样纤维中的氢键和β-折叠网络的程度。