Acquisition of a Liquid Chromatography-Triple Quadrupole/Linear Ion Trap Mass Spe

液相色谱-三重四极杆/线性离子阱质谱仪的采集

• 批准号: 7595631
• 负责人: Indu Kheterpal
• 金额: $ 50万
• 依托单位: LSU PENNINGTON BIOMEDICAL RESEARCH CTR
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-06 至 2011-04-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595631
• 关键词: Advisory Committees; Aging; Biological; Biological Markers; Biomedical Research; Blood - brain barrier anatomy; Carbohydrates; Complex; Core Facility; Detection; Diabetes Mellitus; Disease; Ensure; Fatty Acids; Funding; Health Sciences; Housing; Human Resources; Hybrids; Injection of therapeutic agent; Institution; Ions; Lipids; Liquid Chromatography; Louisiana; Methods; Obesity; Operative Surgical Procedures; Pathway interactions; Peptides; Plasma; Post-Translational Protein Processing; Primates; Protein Analysis; Proteomics; Request for Applications; Research; Research Personnel; Scanning; Schedule; Specificity; System; Technical Expertise; Techniques; Technology; Tissue Sample; Training; Universities; agricultural center; analytical tool; circadian pacemaker; instrument; mass analyzer; mass spectrometer; multiple reaction monitoring; public health relevance; verification and validation

DESCRIPTION (provided by applicant): This application requests funding for the acquisition of a hybrid triple quadrupole/linear ion trap mass spectrometer (4000 Q TRAP; Applied Biosystems) interfaced to a liquid chromatography (LC) system. The new instrument will be housed in the Proteomics Core Facility at the Pennington Biomedical Research Center (PBRC). This instrument is a critical component to support NIH-funded projects of more than ten investigators studying obesity, diabetes and aging. These projects involve detection, identification, characterization and quantification of lipids, fatty acids, metabolites, peptides and protein post-translational modifications. This instrument will allow these investigators to use mass spectrometric (MS) techniques for biomolecular analysis that are currently not available at PBRC or at any neighboring institutions. The facility collaborates with other researchers at Louisiana State University (LSU) Agricultural Center, LSU-Health Sciences Center (HSC) in New Orleans, LSU-HSC in Shreveport, Tulane National Primate Research Center, and small private companies in the South Louisiana region. These investigators will also have access to the technologies offered by this instrument. Today, the analysis of proteins, peptides, lipids, carbohydrates and metabolites using LC-MS/MS methods is commonly performed to identify molecules of biomedical significance. Methods for quantification of these biomolecules in complex matrices such as blood plasma or tissue samples are required for further verification, validation and characterization. The combination of high specificity of the triple quadrupole and high sensitivity of linear ion trap makes 4000 Q TRAP ideal for these projects. The selectivity and limit of quantification achieved using selected and multiple reaction monitoring (SRM and MRM) scan functions are best among all mass analyzers. The 4000 Q TRAP also has MS3 analysis capability which allows for identification and detailed structural characterization of biomolecules in a single injection. Using information dependent acquisition, these scan modes can be used in a variety of combinations including some less conventional combinations such as MRM/Enhanced product ion and MRM/MS3 depending on the application and need for sensitivity, selectivity and/or quantification. This combination of scan modes cannot be achieved by any other instrument. The instrument will be operated and maintained by trained personnel. The Director of the Proteomics Core Facility will schedule daily operation and provide technical expertise to the users. An advisory committee will ensure access to the major users and other NIH-supported investigators and will be responsible for the long term management of the instrument. PUBLIC HEALTH RELEVANCE: The 4000 Q TRAP mass spectrometer will be utilized by the NIH-funded investigators to explore mechanisms leading to obesity, diabetes, and aging and to understand biological pathways controlling circadian clock and blood brain barrier. The unique analytical tools offered by this instrument will also be used to develop biomarkers and to devise therapy for each disease state.

描述（由申请人提供）：本申请要求获得与液相色谱（LC）系统接口的混合三重四极杆/线性离子阱质谱仪（4000 Q TRAP; Applied Biosystems）的资金。新仪器将被安置在彭宁顿生物医学研究中心（PBRC）的蛋白质组学核心设施中。该仪器是支持NIH资助的十多名研究肥胖、糖尿病和衰老的研究人员的项目的关键组成部分。这些项目涉及脂质、脂肪酸、代谢物、肽和蛋白质翻译后修饰的检测、鉴定、表征和定量。该仪器将使这些研究人员能够使用质谱（MS）技术进行生物分子分析，而这些技术目前在PBRC或任何邻近机构都无法使用。该设施与路易斯安那州州立大学（LSU）农业中心、新奥尔良的LSU健康科学中心（HSC）、什里夫波特的LSU-HSC、杜兰国家灵长类动物研究中心以及南路易斯安那州地区的小型私营公司的其他研究人员合作。这些调查人员还将获得该仪器提供的技术。如今，通常使用LC-MS/MS方法分析蛋白质、肽、脂质、碳水化合物和代谢物，以鉴定具有生物医学意义的分子。在复杂的基质，如血浆或组织样品中的这些生物分子的定量方法需要进一步的验证，确认和表征。三重四极杆的高特异性和线性离子阱的高灵敏度相结合，使4000 Q TRAP成为这些项目的理想选择。选择和多反应监测（SRM和MRM）扫描功能实现的选择性和定量限是所有质量分析仪中最好的。4000 Q TRAP还具有MS 3分析能力，可在单次进样中对生物分子进行鉴定和详细的结构表征。使用信息相关采集，这些扫描模式可以以各种组合使用，包括一些不太传统的组合，例如MRM/增强型产物离子和MRM/MS 3，具体取决于应用和对灵敏度、选择性和/或定量的需求。任何其他仪器都无法实现这种扫描模式的组合。仪器将由经过培训的人员操作和维护。蛋白质组学核心设施的主任将安排日常操作，并为用户提供技术专长。一个咨询委员会将确保与主要用户和其他NIH支持的研究人员接触，并负责仪器的长期管理。公共卫生相关性：NIH资助的研究人员将利用4000 Q TRAP质谱仪探索导致肥胖、糖尿病和衰老的机制，并了解控制生物钟和血脑屏障的生物学途径。该仪器提供的独特分析工具也将用于开发生物标志物，并为每种疾病状态设计治疗方法。