HYPOTHALAMIC CONTROL OF LIMB MOVEMENT

了解行为唤醒和运动促进的神经调节，对于理解睡眠觉醒调节的正常控制和运动输出的控制，以及阐明失眠、白天过度嗜睡、快速眼动行为障碍、发作性睡病等临床相关病症的基础具有重要意义。因此，这项研究计划的长期目标是了解下丘脑后部在控制睡眠和觉醒中的作用。提案中包含的实验旨在确定哪些神经递质系统参与下丘脑后丘对皮质和行为（运动）唤醒的调节。它们被设计用来检验以下假设。1)下丘脑后部电微刺激引起的皮层失同步和行为激活是由于细胞体的激活而不是通路纤维的激活。2)谷氨酸释放到下丘脑后部刺激皮层和行为成分的觉醒。组胺释放到下丘脑后部刺激唤醒的关键和行为成分。本提案的第二个目标是向学生介绍生物医学研究。因此，实验被设计成允许大量的学生参与。这项研究将向学生介绍临床重要的睡眠研究领域，以及基本的神经生物学技术。学生获得的技术和技能将为更高级的生物医学科学培训提供坚实的基础和激发兴趣。

Understanding the neural regulation of behavioral arousal and motor facilitation is important to understanding the normal control of sleep- wake regulation and control of motor output, as well as elucidating the basis of such clinically relevant conditions such as insomnia, excessive day time sleepiness, rapid-eye movement behavior disorder and narcolepsy. Consequently, the long-term objective of this research program is to understand the role of the posterior hypothalamus in the control of sleep and arousal. The experiments included in the proposal are designed to determine which neurotransmitter systems are involved in posterior hypothalamic regulation of both cortical and behavioral (motor) arousal. They are designed to test the following hypotheses. 1) The cortical desynchrony and behavioral activation associated with electrical microstimulation of the posterior hypothalamus is due to activation of cell bodies and not fibers of passage. 2) Glutamate release into the posterior hypothalamus stimulates both cortical and behavioral components of arousal. 3) Histamine release into the posterior hypothalamus stimulates both critical and behavioral components of arousal. A secondary objective of this proposal is to introduce students to biomedical research. Consequently, the experiments have been designed to allow substantial student involvement. The research proposed will introduce students to the clinically important field of sleep research, as well as basic neurobiological techniques. The techniques and skills the students acquire will provide a sound base for and stimulate interest in more advanced training in the biomedical sciences.