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Setting a circadian clock: Structural understanding of the ligand-activation model of the circadian evening complex components ELF4 to ELF3

设置生物钟：昼夜节律夜间复合体成分 ELF4 至 ELF3 的配体激活模型的结构理解

基本信息

批准号：
1792522
负责人：
金额：
--
依托单位：
University of York
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2016
资助国家：
英国
起止时间：
2016 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=studentship-1792522
关键词：
Setting circadian clock Structural understanding

项目摘要

The plant circadian clock drives genome-scale transcription to coordinate most of growth and development. EARLY FLOWERING 3 (ELF3) is the key hub for this diurnal signalling. The Davis group recently showed that ELF3 acts as a receptor integrator for the ELF4 ligand, together termed the Evening Complex, to initiate this foundationally important repressor complex. It was also shown that light represses this action, leading to a hypothesis for the mechanism by which increases in light intensity speed up the clock; light signalling acts as a repressor of a repressor. As ELF3 encodes a protein of unknown activity, it has remained enigmatic how it performs its key repressive role. Here we propose a project to unravel a clock-resetting mechanism by ELF3. Using a combination of biochemical, cellular and structural experiments, one would examine the spatial-temporal function of ELF3. One would monitor the global, genome-wide binding of this chromatin-associated factor to define its transcriptional target genes. Next an exploration of the localisation context of where ELF4 activates ELF3, and how light-perception represses this would be examined. Efforts to solve the co-complex of ELF4 and ELF3 will be undertaken and with associated biophysics of complex assembly, how ELF4 association alters ELF3 folding will be elucidated. As ELF3 is known to be bound and repressed by a photoreceptor, activation of ELF3 by ELF4 will be examined in a testable context that supports "photoreceptor-relief" of this key clock factor. Taken together this project is envisaged to provide one mechanistic basis of a long-standing problem in the clock community termed entrainment, where light perception leads to acceleration of periodicity. This is the first ever description of a clock-resetting mechanism in plants.

植物生物钟驱动基因组规模的转录，以协调大部分的生长和发育。早花3号（ELF 3）是这种昼夜信号的关键枢纽。Davis小组最近表明，ELF 3作为ELF 4配体的受体整合剂，一起称为晚间复合物，以启动这种基础重要的阻遏物复合物。研究还表明，光抑制了这种作用，导致了一种假设，即光强度的增加加快了时钟的机制;光信号作为一种阻遏物的阻遏物。由于ELF 3编码一种活性未知的蛋白质，它如何发挥其关键的抑制作用仍然是个谜。在这里，我们提出了一个项目来解开ELF 3的时钟重置机制。使用生物化学，细胞和结构实验的组合，人们将检查ELF 3的时空功能。人们将监测这种染色质相关因子的全局、全基因组结合，以确定其转录靶基因。接下来，将研究ELF 4激活ELF 3的定位背景，以及光感知如何抑制这一点。将努力解决ELF 4和ELF 3的共复合物，并与复合物组装的相关生物物理学，将阐明ELF 4缔合如何改变ELF 3折叠。由于已知ELF 3被感光细胞结合和抑制，因此将在支持该关键时钟因子的“感光细胞-缓解”的可测试背景下检查ELF 4对ELF 3的激活。总而言之，这个项目的设想是提供一个长期存在的问题，在时钟社区称为夹带，其中光的感知导致加速的周期性的机械基础。这是有史以来第一次描述植物中的时钟重置机制。