CORE--IMAGING

图像采集、图像处理和图像分析是基础 所有项目中使用的工具。硬件的可用性和 完成这些任务的软件，以及技术支持的可用性 支持维护和改进现有能力并开发新的能力 有效和及时的处理方法是必不可少的 完成拟议的研究。 核心包括共聚焦显微镜、电子显微镜和 细胞分类，以及支持购买要使用的硬件 为视频图像处理和软件的开发提供便利 从实验图像中提取信息。项目3和项目4 在未来两到三年内面临巨大的发展需求 新的图像处理设备和软件。当前硬件，尽管 对于它设计的任务仍然有效，不再有效 由制造商支持，因此任何组件故障 特定的系统将导致我们的运力大幅下降 来处理图像。在这种情况下，显然不建议这样做 为现有系统制定更多的议定书，特别是在 最近几年发生的技术进步。其中一个 核心基金在下一个供资期间的主要目标将是 为采购新硬件提供技术支持和资源， 将现有功能移植到新平台并开发新的 支持最近开发的实验程序的分析例程。 该计划下一阶段的新需求将是技术支持 用于共焦成像。该大学将提供一台共焦显微镜来 该计划将有足够的访问权限，但技术支持 将不提供操作系统。系统的复杂性， 结合计划的特殊需求(例如，映像的需要 体内和体外的液体灌流微血管系统及其必要性 用于微操作和伴随的压力控制系统) 该计划必须拥有专门的技术专业知识来执行 这些研究。这种支持将由核心提供并可用 所有三个罗切斯特项目。

Image acquisitions, image processing and image analysis are fundamental tools used in all of the projects. The availability of hardware and software to accomplish these tasks, and the availability of technical support to maintain and improve existing capabilities and to develop new processing methodologies is essential for the effective and time completion of the proposed studies. The core includes support for confocal microscopy, electron microscopy and cell sorting, as well as support for the purchase of hardware to be used for video image processing and the development of software to facilitate extraction of information from experimental images. Both projects 3 and 4 face substantial needs in the next two to three years for development of new image processing equipment and software. Current hardware, although still effective for the tasks for which it was designed, is no longer supported by the manufacturer, and so any component failure in a particular system will result in a substantial reduction in our capacity to process images. Given this situation it is clearly inadvisable to develop further protocols for the existing systems, particularly in light of advances in technology that have occurred in recent years. One of the primary objectives for the Core in the next funding period will be to provide the technical support and resources for acquiring new hardware, porting exiting capabilities to the new platform and developing new analysis routines to support recently developed experimental procedures. A new need within the program in the next period will be technical support for confocal imaging. The University will provide a confocal microscope to which the program will have adequate access, but technical support to operate the system will not be provided. The complexity of the system, combined with the special needs of the program (e.g. the need to image fluid-perfused microvascular systems in vivo and in vitro, and the need for micromanipulation and accompanying pressure control systems) make it imperative that the program have dedicated technical expertise to conduct these studies. This support will be provided by the core and be available to all three Rochester projects.