CORE--IMAGING

图像采集、图像处理、图像分析以及荧光信号的检测和解释是所有项目中使用的基本工具。要有效和及时地完成拟议的研究，就必须具备完成这些任务所需的硬件和软件，并提供技术支助，以维持和改进现有能力，并开发新的处理方法。核心包括支持共聚焦显微镜、流式细胞术，支持购买用于视频图像处理的硬件和开发便于提取 实验图像的信息。此外，在当前的应用中，我们建议扩展核心功能，包括全内反射荧光显微镜（TIRFM）。项目2、3和5正在制定新的研究方向，将受益于这一资源，其余两个项目将在较小程度上受益于这些能力的提供。

Image acquisition, image processing, image analysis, and detection and interpretation of fluorescence signals are fundamental tools used in all of the projects. The availability of hardware and software to accomplish these tasks, and the availability of technical support to maintain and improve existing capabilities and to develop new processing methodologies is essential for the effective and timely completion of the proposed studies. The core includes support for confocal microscopy, flow cytometry, and support for the purchase of hardware to be used for video image processing and the development of software to facilitate extraction of information from experimental images. In addition, in the current application we propose to expand core capabilities to include Total Internal Reflectance Fluorescence Microscopy (TIRFM). Projects 2, 3 and 5 are developing new research directions that will benefit from this resource, and the remaining two projects, to a lesser extent, will benefit from the availability of these capabilities.