CORE--IMAGING

图像采集、图像处理、图像分析以及荧光信号的检测和解释是所有项目中使用的基本工具。完成这些任务所需的硬件和软件，以及维持和改进现有能力和发展新的处理方法所需的技术支助，对于有效和及时地完成拟议的研究至关重要。核心包括对共聚焦显微镜、流式细胞术的支持，支持购买用于视频图像处理的硬件和开发用于提取的软件

Image acquisition, image processing, image analysis, and detection and interpretation of fluorescence signals are fundamental tools used in all of the projects. The availability of hardware and software to accomplish these tasks, and the availability of technical support to maintain and improve existing capabilities and to develop new processing methodologies is essential for the effective and timely completion of the proposed studies. The core includes support for confocal microscopy, flow cytometry, and support for the purchase of hardware to be used for video image processing and the development of software to facilitate extraction of information from experimental images. In addition, in the current application we propose to expand core capabilities to include Total Internal Reflectance Fluorescence Microscopy (TIRFM). Projects 2, 3 and 5 are developing new research directions that will benefit from this resource, and the remaining two projects, to a lesser extent, will benefit from the availability of these capabilities.