CORE--IMAGING

图像采集、图像处理和图像分析是基础 所有项目中使用的工具。硬件的可用性， 软件来完成这些任务，以及技术的可用性 支持维持和提高现有能力， 处理方法是必不可少的有效和时间 完成拟议的研究。 核心包括支持共聚焦显微镜，电子显微镜和 细胞分选，以及支持购买硬件使用 用于视频图像处理和软件开发，方便 从实验图像中提取信息。项目3和4 在未来两至三年内， 新的图像处理设备和软件。目前的硬件，虽然 仍然有效的任务，它的设计，不再是 制造商支持，因此， 一个特定的系统将导致我们的能力大大减少 来处理图像在这种情况下， 为现有系统制定进一步的协议，特别是考虑到 近年来发生的技术进步。之一 下一个供资期核心方案的主要目标是 提供获取新硬件的技术支持和资源， 将现有功能移植到新平台，并开发新的 分析程序，以支持最近开发的实验程序。 下一个时期该计划的一个新需求是技术支持 用于共焦成像。大学将提供共聚焦显微镜， 该计划将有足够的访问，但技术支持， 操作系统将不提供。系统的复杂性， 结合程序的特殊需要（例如，需要成像 体内和体外的流体灌注微血管系统，以及 用于显微操作和伴随的压力控制系统）使其 该计划必须有专门的技术专长来进行 这些研究。这种支持将由核心提供， 罗切斯特的三个项目

Image acquisitions, image processing and image analysis are fundamental tools used in all of the projects. The availability of hardware and software to accomplish these tasks, and the availability of technical support to maintain and improve existing capabilities and to develop new processing methodologies is essential for the effective and time completion of the proposed studies. The core includes support for confocal microscopy, electron microscopy and cell sorting, as well as support for the purchase of hardware to be used for video image processing and the development of software to facilitate extraction of information from experimental images. Both projects 3 and 4 face substantial needs in the next two to three years for development of new image processing equipment and software. Current hardware, although still effective for the tasks for which it was designed, is no longer supported by the manufacturer, and so any component failure in a particular system will result in a substantial reduction in our capacity to process images. Given this situation it is clearly inadvisable to develop further protocols for the existing systems, particularly in light of advances in technology that have occurred in recent years. One of the primary objectives for the Core in the next funding period will be to provide the technical support and resources for acquiring new hardware, porting exiting capabilities to the new platform and developing new analysis routines to support recently developed experimental procedures. A new need within the program in the next period will be technical support for confocal imaging. The University will provide a confocal microscope to which the program will have adequate access, but technical support to operate the system will not be provided. The complexity of the system, combined with the special needs of the program (e.g. the need to image fluid-perfused microvascular systems in vivo and in vitro, and the need for micromanipulation and accompanying pressure control systems) make it imperative that the program have dedicated technical expertise to conduct these studies. This support will be provided by the core and be available to all three Rochester projects.