Core--Developmental neurobiology imaging

发育神经生物学成像中心的目标是为大脑皮层突触结构和功能的组装和调节的实验项目提供最先进的设备和技术支持。通过共享技术专长、设备、设施和专业人员，该核心将促进拟议计划项目拨款的不同项目之间的跨项目合作。成像核心将对实验组织进行细胞学和组织学处理，并以定期可靠的方式对计划项目中概述的发育神经生物学项目进行图像分析。通过共享使用核心的项目项目pi发起的新项目也将得到发育神经生物学成像核心的支持。特别是，发育神经生物学共聚焦和电子显微镜水平的所有五个项目妥协的计划项目。在这些项目中，来自胎儿和新生动物的实验神经系统或细胞将进行大量的常规处理，用于显微镜和定量图像分析。此外，从培养的神经元，切片和突触体制剂样品的发光分析将允许快速，高效的谷氨酸释放定量分析。

The objective of the Developmental Neurobiology Imaging Core is to provide state-of-the-art equipment and technical support for experimental projects on the assembly and modulation of synaptic structure and function in the cerebral cortex. By sharing technical expertise, equipment, facilities, and professional staff, this Core will facilitate cross-project collaborations among the different projects of the proposed Program Project Grant The Imaging Core will perform cytological and histological processing of experimental tissues, and perform image analysis in a regular reliable fashion for the development neurobiology projects outlined in the Program Project. New projects initiated through the shared use of the Core by the Program Project P.I.s will also be supported by the Developmental Neurobiology Imaging Core. In particular, the Developmental Neurobiology confocal, and electron microscopic levels for all five projects compromising the Program Project. In each of these projects, experimental nervous system or cells from fetal and neonatal animals will be routinely processed in substantial volumes for microscopy, and quantitative image analysis. In addition, luminometric analysis of samples from cultured neurons, slices, and synaptosomal preparations will allow for rapid, highly efficient quantitative analysis of glutamate release.