Core--Simultaneous laser scanning imaging

描述：同步激光扫描成像核心将允许 调查人员将同时进行电生理记录和 细胞内离子浓度、突触前小泡的单细胞成像 GFP转基因细胞在神经元中的再循环，即细胞形态 脑片上的神经胶质细胞。主要的核心设施将包括一个 激光扫描共聚焦显微镜专用显微镜 用于膜片钳的电生理学设置。核心申请资金用于 01年的传统共聚焦光学显微镜，一个全新的 电生理学装置，包括02年的膜片钳显微镜， 将传统的共焦显微镜升级为双光子激光器 03年的扫描显微镜，并增加了紫外线闪光装置和一个 04年度新增博士后研究员。这一核心很可能是 由十名研究人员使用，他们的研究项目需要同时成像 在电生理记录期间。据预测，核心将是 由一名调查员一次使用10天到两周。

DESCRIPTION: The Simultaneous Laser Scanning Imaging Core will allow investigators to perform simultaneous electrophysiological recordings and single-cell imaging of intracellular ion concentrations, presynaptic vesicle recycling, or cellular morphology of cells transfected with GFP in neurons and glial cells from brain slices. The major core facility will consist of one dedicated laser scanning microscope for confocal microscopy and electrophysiological setup for patch-clamping. The Core requests funding for a conventional confocal light microscope in Year -01, an entire new electrophysiology setup, including a patch-clamp microscope in Year -02, upgrading the conventional confocal light microscope to a two-photon laser scanning microscope in Year -03, and addition of UV flash unit and an additional postdoctoral fellow in Year -04. This Core will most likely be used by ten investigators whose research projects demand simultaneous imaging during electrophysiological recording. It is predicted that the Core will be utilized by one investigator for 10 days to two weeks at a time.