Mechanisms of post-transcriptional regulation of MeCP2

描述(申请人提供)：本申请的目标是揭示MeCP2基因异常大的3‘非翻译区的形成和功能调节所涉及的序列和因素。这个大约8.5kb的3‘非编码区可能包含在转录后水平调节基因表达的元件。首先，研究人员将确定在MeCP2基因的选择性多聚腺苷化过程中发挥作用的辅助调控元件和因素，该过程以组织和发育特有的方式决定3‘UTR的大小。其次，他们将检验这一假设，即长3‘非编码区在决定MeCP2 mRNA的差异稳定性或翻译方面发挥作用。这些研究代表了MeCP2表达不足的区域，这些区域可能对Rett综合征观察到的组织特异性效应具有关键影响。

DESCRIPTION (provided by applicant): The goal of this application is to uncover the sequences and factors involved in the regulation of the formation and function of the unusually large 3' untranslated region of the MeCP2 gene. This approximately 8.5 kb 3' UTR likely contains elements that regulate gene expression at the post-transcriptional level. First, the investigators will identify auxiliary regulatory elements and factors that play a role in alternative polyadenylation of the MeCP2 gene, the process that determines the size of the 3' UTR in a tissue- and developmental-specific fashion. Second, they will test the hypothesis that the long 3' UTR plays a role in determining differential stability or translation of the MeCP2 mRNA. These studies represent under-explored areas of MeCP2 expression that may have a critical influence in the tissue-specific effects observed in Rett syndrome.