Mechanisms of MeCP2 gene expression regulation

MeCP2基因表达调控机制

• 批准号: 7184526
• 负责人: CAROL S LUTZ
• 金额: $ 7.79万
• 依托单位: UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-15 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7184526
• 关键词: 3' Untranslated Regions; 5' Untranslated Regions; Accounting; Address; Affect; Age-Months; Area; Attention; Autistic Disorder; Binding; Binding Proteins; Bioinformatics; Brain; Candidate Disease Gene; Cell Line; Chromatin; Chromosome Mapping; Code; Conserved Sequence; Development; Diagnosis; Disease; Elements; Exclusion; Exons; Female; Figs - dietary; Frequencies; Functional RNA; Gene Expression; Gene Expression Regulation; Genes; Genetic Transcription; Goals; Hand; In Vitro; Incidence; Individual; Laboratories; Link; Messenger RNA; Methyl-CpG-Binding Protein 2; Microcephaly; Modeling; Mus; Mutation; Names; Neurodevelopmental Disorder; Neurons; Open Reading Frames; Patients; Phenotype; Play; Poly A; Polyadenylation; Principal Investigator; Process; Protein Isoforms; RNA; RNA Stability; Regulation; Reporter; Reporting; Research; Research Personnel; Rett Syndrome; Role; Schizophrenia; Signal Transduction; Speech; Symptoms; Syndrome; System; Tissues; Transcript; Translations; Untranslated Regions; Work; Xq28; base; in vivo; insight; knockout gene; mRNA Stability; novel; relating to nervous system; size; transmission process

DESCRIPTION (provided by applicant): The goal of this application is to determine the post-transcriptional mechanisms of gene expression control of the MeCP2 gene. The principal investigator will delineate alternative polyadenylation of MeCP2, the process that determines the size of the 3' UTR in a tissue-and developmentally-specific fashion. She will also investigate the role that mRNA stability may play in MeCP2 expression regulation. These studies will be performed in vivo in a variety of cell lines with special attention to neural cells. The investigator will then turn to established in vitro systems using these cell lines in order to investigate mechanisms of regulation by alternative polyadenylation and differential RNA stability. These studies represent an under-explored area of research on MeCP2 gene expression that may have a critical influence in the tissue-specific effects observed in Rett syndrome.

描述（由申请人提供）：本申请的目的是确定MeCP2基因表达控制的转录后机制。首席研究员将描述MeCP2的选择性聚腺苷化，该过程以组织和发育特异性的方式决定3' UTR的大小。她还将研究mRNA稳定性在MeCP2表达调控中可能发挥的作用。这些研究将在多种细胞系中进行，特别关注神经细胞。然后，研究者将转向使用这些细胞系建立的体外系统，以研究通过选择性聚腺苷酸化和差异RNA稳定性进行调节的机制。这些研究代表了MeCP2基因表达研究的一个未被充分探索的领域，该领域可能对Rett综合征中观察到的组织特异性效应具有关键影响。