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Mechanisms of post-transcriptional regulation of MeCP2

MeCP2转录后调控机制

基本信息

批准号：
6629439
负责人：
CAROL S LUTZ
金额：
$ 7.78万
依托单位：
UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-07-01 至 2005-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6629439
关键词：
CpG islands RNase protection assay Rett syndrome biological signal transduction cell line gene expression genetic regulation genetic regulatory element messenger RNA molecular cloning northern blottings nucleic acid sequence polyadenylate posttranscriptional RNA processing site directed mutagenesis tissue /cell culture transcription factor

项目摘要

DESCRIPTION (provided by applicant): The goal of this application is to uncover the sequences and factors involved in the regulation of the formation and function of the unusually large 3' untranslated region of the MeCP2 gene. This approximately 8.5 kb 3' UTR likely contains elements that regulate gene expression at the post-transcriptional level. First, the investigators will identify auxiliary regulatory elements and factors that play a role in alternative polyadenylation of the MeCP2 gene, the process that determines the size of the 3' UTR in a tissue- and developmental-specific fashion. Second, they will test the hypothesis that the long 3' UTR plays a role in determining differential stability or translation of the MeCP2 mRNA. These studies represent under-explored areas of MeCP2 expression that may have a critical influence in the tissue-specific effects observed in Rett syndrome.

描述（由申请人提供）：本申请的目的是揭示参与调节MeCP 2基因异常大的3'非翻译区的形成和功能的序列和因子。该约8.5kb的3' UTR可能含有在转录后水平调节基因表达的元件。首先，研究人员将确定辅助调控元件和因子，这些元件和因子在MeCP 2基因的替代性多聚腺苷酸化中发挥作用，该过程以组织和发育特异性方式决定3' UTR的大小。其次，他们将检验长3'UTR在决定MeCP2 mRNA的差异稳定性或翻译中起作用的假设。这些研究代表了未充分探索的MeCP2表达领域，这些领域可能对Rett综合征中观察到的组织特异性效应产生关键影响。