Development of inhibitors for a novel interaction site between translocase MraY and the lysis protein E from bacteriophage X174

开发易位酶 MraY 和噬菌体 X174 裂解蛋白 E 之间新型相互作用位点的抑制剂

• 批准号: 1796354
• 金额: --
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1796354
• 关键词: Development; inhibitors; novel; interaction; site

The project will investigate a protein-protein interaction site between translocase MraY and antibacterial protein E from bacteriophage phiX174. Novel peptide inhibitors will be synthesised for this interaction site, based an an Arg-Trp-x-x-Trp sequence motif identified previously. Peptidomimetic analogues of this motif will be synthesised later in the project. The synthetic compounds will be assyed as inhibitors of translocase MraY using a fluorescence assay, and the compounds will be tested for antibacterial activity against a panel of Gram-negative and Gram-positive bacteria.

该项目将研究转位酶mray和噬菌体phiX174中的抗菌蛋白E之间的蛋白质-蛋白质相互作用位点。基于先前确定的Arg-Trp-x-x-Trp序列基序，将合成针对该相互作用位点的新型多肽抑制剂。这个主题的模拟多肽的类似物将在项目的后期合成。合成的化合物将使用荧光分析作为转位酶mray的抑制剂，并将测试化合物对一组革兰氏阴性和革兰氏阳性细菌的抗菌活性。