Isolation and characterisation of bacteriophages against avian Escherichia coli carrying multi-drug resistant (MDR) plasmids

一般背景：携带耐多药耐药性质粒的大肠杆菌等细菌性病原体的抗生素耐药性被认为是对全球健康的最大威胁之一，估计每年造成70万人死亡。不幸的是，由于抗生素耐药性的增加，控制这些病原体的策略受到阻碍，因此有必要研究替代治疗方法。噬菌体（噬菌体）是一种只感染并杀死细菌的病毒，自20世纪80年代以来，西方一直在讨论将其作为抗生素化疗的替代品。项目背景：除了通过直接感染杀死病原体外，噬菌体还可以作为一种工具，利用噬菌体附着在AMR质粒的结合菌毛上，减少MDR质粒的携带。我们有DTP轮换部分的初步数据（Maluping, Atterbury, Barrow, Jones 2017）。从最初的15株噬菌体分离株中鉴定出4株含有抗性质粒pFlacTn3的特异性靶向大肠杆菌噬菌体。（Maluping， R.，旋转数据Barrow et al. 2017）。这些噬菌体可用于特异性靶向携带AMR细菌的质粒，减少AMR，为AMR细菌感染的治疗提供支持。作为这些细菌的一部分，最初的EM表征表明，这些噬菌体都有尾部，很可能被归类为肌病毒科的成员（Maluping， R.，轮换数据Barrow et al. 2017）。

General background: Antibiotic resistance in bacterial pathogens such as Escherichia coli carrying MDR plasmids is considered to be one of the greatest threats to global health, and responsible for an estimated 700,000 deaths per year. Unfortunately, strategies for the control of such pathogens have been impeded by increasing antibiotic resistance, so there is a need to investigate alternative treatments. Bacteriophage (phage) are viruses that infect and kill only bacteria, and have been mooted as an alternative to antibiotic chemotherapy in the West since the 1980s.Project background: In addition to killing pathogens by direct infection, phage can also be used as a tool to reduce the carriage of MDR plasmids using phage attach to conjugative pili of the AMR plasmids. We have preliminary data a part of the DTP rotation (Maluping , Atterbury, Barrow, Jones 2017). Four phage were identified phage which specifically targeted E. coli contain the resistance plasmid pFlacTn3 from an initial pool of 15 phage isolates. (Maluping, R., rotation data Barrow et al. 2017). These phage could be used to specifically target the plasmid bearing AMR bacteria and reduce AMR to support treatment of AMR bacterial infections. Initial EM characterization as part of these bacteria indicated that these phage were all tailed and most likely classified as members of the Myoviridae (Maluping, R., rotation data Barrow et al. 2017).