Biomarkers of Oxidative Stress Study

大鼠血浆中的抗氧化剂是作为一项全面的多实验室验证研究的一部分进行测量的，该研究旨在寻找氧化应激的非侵入性生物标志物。在这项初步研究中，研究了四氯化碳中毒的动物模型。 时间（2、7和16 h）和剂量（120 mg/kg和1200 mg/kg，i.（第30页）研究了CCl4对α-生育酚、抗坏血酸、谷胱甘肽（GSH和GSSG）、尿酸和总抗氧化能力的血浆水平的依赖性影响，以确定CCl4的氧化作用是否会导致血浆中抗氧化剂的损失。CCl4处理大鼠的α-生育酚浓度降低。 然而，由于胆固醇和甘油三酯同时降低，无法将α-生育酚氧化与全身脂质变化分离。在最早的时间点，抗坏血酸水平较高，GSH与GSSG的比值普遍下降，尿酸保持不变。 总抗氧化能力没有显着的变化，除了16小时后，高剂量，当它增加。这些结果表明，由于肝功能障碍和肝细胞破裂导致的血浆变化阻碍了血浆抗氧化状态的任何潜在变化的检测。

Antioxidants in the blood plasma of rats were measured as part of a comprehensive, multilaboratory validation study searching for non-invasive biomarkers of oxidative stress. For this initial study an animal model of CCl4 poisoning was studied. The time-(2, 7 and 16 h) and dose-(120 mg/kg and 1200 mg/kg, i. p.) dependent effects of CCl4 on plasma levels of alpha-tocopherol, ascorbic acid, glutathione (GSH and GSSG), uric acid and total antioxidant capacity were investigated to determine whether the oxidative effects of CCl4 would result in losses of antioxidants from plasma. The alpha-tocopherol concentration was decreased in CCl4-treated rats. However, due to concomitant decreases in cholesterol and triglycerides, it was impossible to dissociate oxidation of alpha-tocopherol from generalized lipid changes. Ascorbic acid levels were higher with treatment at the earliest time point; the ratio of GSH to GSSG generally declined, and uric acid remained unchanged. Total antioxidant capacity showed no significant change except for 16 h after the high dose, when it was increased. These results suggest that plasma changes due to liver malfunction and rupture of liver cells impede detection of any potential changes in the antioxidant status of the plasma.