Superresolution and correlative microscopy to study cell adhesion on nanopatterned substrates

超分辨率和相关显微镜研究纳米图案基底上的细胞粘附

• 批准号: 1804171
• 金额: --
• 依托单位: University of Glasgow
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1804171
• 关键词: Superresolution; correlative; microscopy; study; cell

As cells adhere to surfaces, they form focal adhesions which link the cells mechanically to their surroundings but also provide important signalling too. With the invention of superresolution microscopy it has become possible to study the proteins involved in these adhesions at a molecular level. The improvement in detail is typically 10 times with a resolution of about 20 nm. At the same time semiconductor technologies have made it possible to control patterns with a precision similar to the size of the proteins. This opens up the possibilities to potentially manipulate the adhesions at a molecular level. These interactions can be visualised with a combination of superresolution and scanning electron microscopy, known as correlative microscopy. This project will use advanced microscopy and genetically engineered cells to investigate the adhesion formation on a range of nanopatterned surfaces. The aim will be to correlate the formation of the focal adhesions with the design parameters with a view to produce engineered materials for regenerative medicine.

当细胞粘附在表面上时，它们形成局部粘附，将细胞机械地连接到周围环境，但也提供重要的信号。随着超分辨率显微镜的发明，在分子水平上研究这些粘连中涉及的蛋白质已经成为可能。在分辨率约为20 nm的情况下，细节的改善通常为10倍。与此同时，半导体技术使控制模式的精度与蛋白质的大小相似成为可能。这为在分子水平上操纵粘连提供了可能性。这些相互作用可以通过超分辨率和扫描电子显微镜的组合来可视化，称为相关显微镜。该项目将使用先进的显微镜和基因工程细胞来研究一系列纳米图案表面上的粘附形成。目的是将焦点粘连的形成与设计参数相关联，以生产用于再生医学的工程材料。