in situ Epigenetic Profiling of Single Cells in Kidney

肾脏单细胞的原位表观遗传分析

• 批准号: 10645605
• 负责人: Joshua Vaughan
• 金额: $ 22.9万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10645605
• 关键词: Address; Adult; Aging; American; Blood; Cells; Cessation of life; Chromatin; Chronic Kidney Failure; Complex; Correlative Study; Cultured Cells; DNA; DNA Methylation; Dialysis procedure; Disease; Dissociation; Electrolyte Balance; End stage renal failure; Endothelial Cells; Epigenetic Process; Evaluation; Face; Fluorescent in Situ Hybridization; Generations; Genome; Goals; Health Expenditures; Heterochromatin; Histones; Image; In Situ; Individual; Injury; Kidney; Kidney Diseases; Maps; Measures; Methods; Microscopy; Molecular; Molecular Conformation; Mus; Organ; Phenotype; Physiological; Physiology; Population; Post-Translational Protein Processing; Publishing; Renal function; Research Personnel; Risk; Role; Specimen; Stains; Techniques; Technology; Time; Tissues; Transplantation; Use of New Techniques; Work; base; blood pressure regulation; design; epigenetic profiling; field study; genome-wide; genomic locus; glomerular basement membrane; healthy aging; histone modification; human old age (65+); innovation; insight; kidney cell; kidney imaging; microscopic imaging; molecular phenotype; mouse model; nanoscale; novel; podocyte; senescence; single cell analysis; superresolution microscopy; tool; ultra high resolution; wasting; whole genome

Project Summary/Abstract The goal of this proposal is to develop and validate two tools for in situ analysis of epigenetic state and chromatin organization in single cells in kidney tissue. The first tool is based on our recently developed technology SCEPTRE (Single-Cell Evaluation of Post TRanslational Epigenetic encoding) established for cultured cells that will be adapted for use with kidney tissues in order to directly resolve and quantify histone modifications at specific DNA loci in situ in single cells. The second tool is a new method called APT-FISH (Amplified Probes from Tagmentation Fluorescence in situ Hybridization) that is designed to profile changes to open chromatin. Each of these tools will be used concurrently with super-resolution expansion microscopy imaging in order to directly relate nanoscale renal physiology to chromatin or epigenetic state and they will be applied to the study of aging and senescence in kidney tissue.

项目总结/文摘