in situ Epigenetic Profiling of Single Cells in Kidney - Kim Diversity Supplement 2023

肾脏单细胞原位表观遗传分析 - Kim Diversity Supplement 2023

基本信息

批准号：
10849346
负责人：
Joshua Vaughan
金额：
$ 5.8万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-08-01 至 2025-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10849346
关键词：
Address Adult Aging American Blood Cell Separation Cells Cessation of life Chromatin Chronic Kidney Failure Complex Correlative Study Cultured Cells DNA DNA Methylation Dialysis procedure Disease Dissociation Electrolyte Balance End stage renal failure Endothelial Cells Epigenetic Process Evaluation Face Fluorescent in Situ Hybridization Generations Genome Goals Health Expenditures Heterochromatin Histones Image Immunofluorescence Immunologic In Situ Individual Injury Kidney Kidney Diseases Label Maps Measures Methods Microscopy Molecular Molecular Conformation Mus Organ Phenotype Physiological Physiology Population Post-Translational Protein Processing Publishing Renal function Research Personnel Risk Role Specimen Stains Techniques Time Tissues Transplantation Use of New Techniques Work base blood pressure regulation epigenetic profiling field study genome-wide genomic locus glomerular basement membrane healthy aging histone modification human old age (65+)innovation insight kidney cell kidney imaging molecular phenotype mouse model nanoscale novel podocyte senescence single cell analysis superresolution imaging superresolution microscopy tool ultra high resolution wasting whole genome

项目摘要

Project Summary/Abstract We have recently developed a microscopy-based method that, for the first time, enables interrogation of histone modifications at single loci in single cells. This method is termed SCEPTRE (for Single-Cell Evaluation of Post TRanslational Epigenetic encoding) and utilizes super-resolution imaging to directly resolve and quantify histone modifications at specific DNA or gene loci that are labeled by immunofluorescence and DNA fluorescence in situ hybridization (FISH), respectively. We propose to develop SCEPTRE, a method currently in use with cultured or isolated cells, for epigenetic profiling of kidney tissues and to use SCEPTRE to study healthy aging and senescence in mouse kidney tissues.

项目摘要/摘要我们最近开发了一种基于显微镜的方法，该方法首次质疑组蛋白单个细胞中的单基因座进行修饰。此方法称为权杖（用于邮政的单细胞评估翻译表观遗传编码）并利用超分辨率成像直接解析和量化组蛋白特定DNA或基因基因座的修饰，该基因座被免疫荧光和DNA荧光原位标记分别杂交（鱼）。我们建议开发权杖，这是一种目前与文化或分离的细胞，用于肾脏组织的表观遗传分析，并使用权权者研究健康的衰老和小鼠肾脏组织中的衰老。