Antioxidant Activity of Iron-Amino Acid-Chelator Complexes

铁-氨基酸-螯合剂复合物的抗氧化活性

• 批准号: 6432630
• 负责人: EARL R STADTMAN
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6432630
• 关键词: antioxidants; chelating agents; chemical aggregate; chemical reaction; cytotoxicity; free radical scavengers; growth media; histidine; iron compounds; metal complex; oxidative stress; spectrometry; tissue /cell culture

Taking advantage of the unique spectral characteristics of ternary complexes formed between amino acids, ferrous iron, and ferrozine, studies on the antioxidant activity of such complexes were continued. Decomposition of the ternary complex was found to occur by two independent pathways: (i) a non-oxidative pathway leading to formation of a ferrous iron-ferrozine complex and (ii) an oxidative pathway leading to ferric iron and colorless products. These processes were associated with occurrence of an isosbestic point (i.p.) whose wavelength varied from 601 to 574 nano meters, depending on experimental conditions. Results of simulation experiments, confirmed by direct analyses, demonstrate that the wavelength of the i.p. can be used to determine the fractions of ternary complex that are decomposed by the oxidative and non-oxidative pathways.

利用氨基酸、亚铁和亚铁嗪之间形成的三元络合物的独特光谱特性，继续研究此类络合物的抗氧化活性。 发现三元复合物的分解通过两个独立的途径发生：（i）导致形成亚铁-亚铁嗪复合物的非氧化途径和（ii）导致三价铁和无色产物的氧化途径。 这些过程与等吸光点（i. p.）其波长根据实验条件从601纳米变化到574纳米。 通过直接分析证实的模拟实验的结果表明，IP的波长可用于确定由氧化和非氧化途径分解的三元复合物的分数。