MUSCLE PROTEIN BIOPHYSICS VIA ATOMIC FORCE MICROSCOPY

肌肉生理学和肌肉病理学是NIAMS和NHLBI等国际组织使命下的重要研究课题。在与NIAMS研究人员的持续合作中，原子力显微镜（AFM）和相关的生物物理方法正在应用于研究肌肉蛋白质，如titin （3-4 MDa的大量肽链）和nebulin （600-900kDa）。他的工作包括与他人共同撰写了一篇关于AFM和激光光阱（LOT）纳米技术在肌苷研究中的应用的综合综述，以及由此产生的对肌苷生物物理特性和肌肉生理病理意义的理解。原子力显微镜的测量和解释的分子弹性的titin motif，称为PEVK区域，也在继续。星云结构和模折叠能量学的原子力显微镜研究和数据解释以及原子力显微镜仪器的进步是其他活动领域

Muscle physiology and muscle pathologies are important research subjects under the missions of ICs such as NIAMS and NHLBI. In a continuing collaboration with NIAMS researchers, atomic force microscopy (AFM) and related biophysical methods are being applied to investigate muscle proteins such as titin (a massive peptide chain of 3-4 MDa) and nebulin (600-900kDa). The work includes coauthoring a comprehensive review of AFM and laser optical trap (LOT) nanotechnologies as applied to titin research and the resulting understandings on titin biophysical properties and implications for muscle physiology and pathology. AFM measurements and interpretations of the molecular elasticity of a titin motif, termed PEVK region, is also continuing. AFM investigations of nebulin structure and modular folding energetics and data interpretations and AFM instrument advancement are other areas of activity and achievements.