FUNCTION GENOMICS OF CRITICAL ILLNESS

危重疾病的功能基因组学

• 批准号: 6414062
• 负责人: ROBERT L DANNER
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6414062
• 关键词: adult respiratory distress syndrome; clinical research; functional /structural genomics; human subject; laboratory mouse; laboratory rat; molecular biology information system; multiple organ failure; myocardium disorder; septic shock

Critical illness syndromes, such as acute respiratory distress syndrome (ARDS), septic shock, myocardial depression, and multiple organ failure all share the hypothesis that the host response plays a central pathogenic role. In this project, CCMD has established the infrastructure necessary to define these pathogenic host responses at the level of gene expression across thousands of mRNA transcripts, simultaneously. This technology will be used to create a large critical illness functional genomics database using in vitro models, small animal (rat and mouse) models, endotoxin-challenged volunteers, and ultimately critically ill patients.

危重疾病综合征，如急性呼吸窘迫综合征（ARDS）、感染性休克、心肌抑制和多器官衰竭，都有一个共同的假设，即宿主反应起核心致病作用。在这个项目中，CCMD已经建立了必要的基础设施，可以同时在数千个mRNA转录物的基因表达水平上定义这些致病性宿主反应。这项技术将用于创建一个大型的危重疾病功能基因组数据库，包括体外模型、小动物（大鼠和小鼠）模型、内毒素挑战志愿者，以及最终的危重患者。