Cell-cell Interactions Between Oral Actinomyces and other Bacteria

口腔放线菌与其他细菌之间的细胞间相互作用

• 批准号: 6432000
• 负责人: PAUL E KOLENBRANDER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6432000
• 关键词: Actinomyces; Bacillus subtilis; Fusobacterium nucleatum; Helicobacter; Streptococcus; adenosinetriphosphatase; adhesin; bacterial DNA; bacterial genetics; carbohydrate transport; dental plaque; lactose; membrane proteins; microorganism culture; microorganism interaction; nonblood lipoprotein; nucleic acid sequence; operon; oral bacteria; plasmids; site directed mutagenesis; transposon /insertion element

Our focus is on contact-induced gene expression in oral streptococci and the spatial organization of these bacteria, oral actinomyces and other oral bacteria in biofilm communities. Dental plaque is a biofilm community, which is characterized by a complex and highly organized set of interactions among oral bacteria as well as between bacteria and human oral tissue. This initiative is centered on the bacteria known to be early colonizers of a freshly cleaned tooth surface. They represent a significant portion of the bacterial activity in biofilms formed in the first four to eight hours in vitro on saliva-coated glass surfaces and in vivo on removable human enamel chips. We are also investigating the oral fusobacteria because they interact with the broadest variety of oral bacteria including early colonizers, late colonizers and potential periodontal pathogens and, therefore, are thought to be major contributors to dental plaque organization. The overall focus of this project is to investigate cell adherence molecules, cell signaling molecules, gene expression and gene transfer among bacteria within human oral biofilms and to relate these events to bacterial community architecture. Our long-range goal is to understand the molecular mechanisms of cellular communication and their relationship to the development and establishment of dental plaque and colonization of the host epithelial cells.

我们的重点是口腔链球菌中接触诱导的基因表达以及这些细菌、口腔放线菌和生物膜群落中其他口腔细菌的空间组织。牙菌斑是一个生物膜群落，其特征是口腔细菌之间以及细菌与人体口腔组织之间存在复杂且高度组织的相互作用。 这一举措的重点是已知在刚清洁的牙齿表面早期定殖的细菌。 它们代表了在体外唾液涂层玻璃表面和体内可移除的人牙釉质芯片上前四到八小时内形成的生物膜中细菌活性的重要部分。 我们还在研究口腔梭杆菌，因为它们与最广泛的口腔细菌相互作用，包括早期定植菌、晚期定植菌和潜在的牙周病原体，因此被认为是牙菌斑组织的主要贡献者。 该项目的总体重点是研究人类口腔生物膜内细菌之间的细胞粘附分子、细胞信号分子、基因表达和基因转移，并将这些事件与细菌群落结构联系起来。 我们的长期目标是了解细胞通讯的分子机制及其与牙菌斑的发育和建立以及宿主上皮细胞定植的关系。