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Lactoferrin as an Adjuvant for Cellular Immunity

乳铁蛋白作为细胞免疫佐剂

基本信息

批准号：
6552378
负责人：
JEFFREY K ACTOR
金额：
$ 10万
依托单位：
PHARMAREVIEW CORPORATION
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-09-15 至 2004-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6552378
关键词：
Bacillus Calmette Guerin vaccine cellular immunity immunologic substance development /preparation immunomodulators interleukin 12 interleukin 15 laboratory mouse lactoferrin tumor necrosis factor alpha vaccine development veterinary medicine

项目摘要

DESCRIPTION (provided by applicant): The overall goal of this project is to develop bovine and human Lactoferrins as an adjuvant to augment antibody and cell mediated immunity. We will specifically address the efficacy of Lactoferrin as an adjuvant to augment efficacy of BCG vaccination to subsequent challenge with virulent Mycobacterium tuberculosis. Improved nontoxic adjuvants, especially adjuvants capable of inducing (CM are needed for research use and for development of human and veterinary vaccines. The only adjuvant licensed for human use, alum, effectively enhances antibody formation, but is seldom effective for inducing CMI. Other adjuvants such as saponin and Complete Freund's that do induce CMI are much too toxic for use in humans and are being progressively restricted in animals. Lactoferrin is different from microbial derivative adjuvants, and from cytokines and growth factors; it is an effector molecule that directly participates in host defenses. Available evidence indicatesLactoferrin participates in the induction of immune responses, especially CMI. Preliminary studies demonstrate that lactoferrin as an adjuvant enhances delayed type hypersensitivity (DTH) in mice in a dose dependent fashion and has low toxicity. Furthermore, it induces IL-12, IL-15 and TNF-a production by macrophages thereby generating a local environment likely to push immune responses towards development of CMI.

描述（由申请人提供）：该项目的总体目标是开发牛和人乳铁蛋白作为增强抗体和细胞介导的免疫的佐剂。我们将特别讨论乳铁蛋白作为佐剂的功效，以增强卡介苗疫苗接种以应对随后的强毒力结核分枝杆菌攻击的功效。改进的无毒佐剂，特别是能够诱导（CM）的佐剂用于研究用途以及开发人用和兽用疫苗。明矾是唯一被许可用于人类的佐剂，可有效增强抗体形成，但对诱导 CMI 很少有效。其他佐剂，如皂苷和完全弗氏佐剂，可诱导 CMI，但毒性太大，不适合用于人类，因此正在逐渐受到限制在动物中。乳铁蛋白不同于微生物衍生佐剂，也不同于细胞因子和生长因子；它是一种直接参与宿主防御的效应分子。现有证据表明乳铁蛋白参与免疫反应的诱导，尤其是 CMI。初步研究表明，乳铁蛋白作为佐剂以剂量依赖性方式增强小鼠迟发型超敏反应（DTH），并且具有低毒。此外，它诱导巨噬细胞产生 IL-12、IL-15 和 TNF-a，从而产生可能推动免疫反应促进 CMI 发展的局部环境。