Nucleic Acid Binding & Amplifications Matrices

核酸结合

• 批准号: 6511640
• 负责人: Jeffrey T. Ives
• 金额: $ 20.72万
• 依托单位: XTRANA, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-15 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6511640
• 关键词: biomaterial development /preparation; metal oxide; molecular assembly /self assembly; nanotechnology; nucleic acid amplification techniques

DESCRIPTION (provided by applicant): Nanotechnology can potentially transform molecular diagnostics from its current state of manual procedures or expensive equipment to routine, low cost assays for a broad range of microbial and cellular panels. As an initial step, this project will create nanoengineered materials that will uniquely simplify the process of nucleic acid preparation and amplification. The materials will serve as components in microfluidic "lab on a chip" devices, enhancing the devices' current capabilities, but more importantly, allowing advanced designs to be fully integrated over an array of parallel targets. This project will combine Xtrana's proprietary metal oxides with the self-assembled, nanofabrication technology of Sandia National Laboratory (SNL) to create microscale frits that irreversibly bind nucleic acids. The DNA and RNA can then be enzymatically amplified while on the surface, without the inefficiencies and complexities of elution. The operational and multiplexing capabilities associated with these frits are key to routine, multi-target diagnostics. This project is a model collaboration between Xtrana's materials and molecular diagnostics expertise and SNL's innovative self-assembly methods to create membranes and other structures containing ordered arrays of nanoscale, metal oxide pores. Phase II will further develop and test the materials in advanced microfluidic devices to identify infectious agents and cellular polymorphisms. PROPOSED COMMERCIAL APPLICATION: Due to the ubiquitous presence of nucleic acids and the broad-based utility of an effective method of preparing DNA and RNA, the described materials would have commercial value in many areas. A list of the most prominent would include health care, biowarfare defense, food and water safety, environmental monitoring, screening for genetic predispositions, tissue typing, forensics, and parentage verification.

描述（由申请人提供）：纳米技术可以潜在地改变 分子诊断从其目前的手动程序或昂贵的状态 设备的常规，低成本的测定范围广泛的微生物和 蜂窝板作为第一步，该项目将创建纳米工程 将独特地简化核酸制备过程的材料 和放大。这些材料将作为微流体实验室的组件 片上”器件，增强了器件的当前能力，但更多 重要的是，允许先进的设计完全集成在一系列 平行目标该项目将联合收割机Xtrana的专有金属氧化物 利用桑迪亚国家实验室的自组装纳米纤维技术 实验室（SNL）创建不可逆地结合核酸的微型熔块 acids.然后，DNA和RNA可以在酶促扩增的同时进行酶促扩增。 表面，没有洗脱的低效和复杂性。的 与这些熔块相关的操作和多路复用能力是关键 常规的多目标诊断这个项目是一个典型的合作 Xtransa的材料和分子诊断专业知识与SNL的 创造膜和其他结构的创新自组装方法 含有纳米级金属氧化物孔的有序阵列。二期将 进一步开发和测试先进微流体装置中的材料， 鉴定感染因子和细胞多态性。 拟定商业应用： 由于核酸的普遍存在和制备DNA和RNA的有效方法的广泛应用，所述材料将在许多领域具有商业价值。其中最突出的包括医疗保健、生物战防御、食品和水安全、环境监测、遗传易感性筛查、组织分型、法医学和亲子鉴定。