TIME RESOLVED PROTEIN FOLDING USING LAUE WHITE BEAM SMALL ANGLE XRAY SCATTERING

使用 Laue 白束小角 X 射线散射进行时间分辨蛋白质折叠

• 批准号: 6437721
• 负责人: SEBASTIAN DONIACH
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6437721
• 关键词: bioimaging /biomedical imaging; biological products; biomedical equipment development; biomedical resource; proteins; radiography; structural biology; technology /technique

In addition to exploring "Laue" SAXS, i.e. using very broad-band quasi-white radiation, we have evaluated the use of a narrower broad-band radiation setup using multilayer monochromators. The use of the these multilayers (200 eV bandpass) on BL4-2 resulted in a 20-fold increase of x-ray flux over the normal Si(111) monochromator. This flux increase resulted in better time resolution for time-resolved SAXS protein folding studies. SAXS is the only experimental probe which can give overall size and shape indication of the conformational state of a protein. Time-resolved SAXS studies on the refolding of lysozyme was performed (manuscript in preparation). We have performed preliminary experiments using SAXS to study protein folding initiated with a continuous flow mixer. Control studies show that reasonable radius of gyration measurements can be obtained with this method.

除了探索“劳厄”SAXS，即使用非常宽的波段 准白色辐射，我们已经评估了使用较窄的 使用多层单色器的宽带辐射设置。 使用 在BL 4 -2上的这些多层膜（200 eV带通）中， 20-X射线通量比正常Si（111）单色器增加一倍。 这种通量增加导致更好的时间分辨率， 时间分辨SAXS蛋白质折叠研究。 SAXS是唯一 实验探头，可以给出整体尺寸和形状的指示， 蛋白质的构象状态。 时间分辨小角X射线散射研究 进行溶菌酶的重折叠（手稿在准备中）。 我们已经进行了初步的实验，使用SAXS研究蛋白质 用连续流动混合器开始折叠。 对照研究显示 合理的回转半径测量可以通过 该方法