TIME RESOLVED PROTEIN FOLDING USING LAUE WHITE BEAM SMALL ANGLE XRAY SCATTERING

除了探索“Laue”SAXS，即使用非常宽的频带 准白光辐射，我们已经评估了使用更窄的 使用多层单色仪的宽带辐射设置。它的用途 在BL4-2上的这些多层膜(200 eV带通)导致了 X射线通量比普通的Si(111)单色仪提高了20倍。 这种通量的增加导致了更好的时间分辨率 时间分辨SAXS蛋白质折叠研究。SAXS是唯一的 可给出整体尺寸和形状指示的实验探头 蛋白质的构象状态。时间分辨小角X射线光谱分析研究 对溶菌酶进行复性(手稿准备中)。 我们已经用SAXS进行了初步的实验来研究蛋白质 用连续流动搅拌机开始折叠。控制研究表明 合理的回转半径测量可以用以下方法获得 这种方法。

In addition to exploring "Laue" SAXS, i.e. using very broad-band quasi-white radiation, we have evaluated the use of a narrower broad-band radiation setup using multilayer monochromators. The use of the these multilayers (200 eV bandpass) on BL4-2 resulted in a 20-fold increase of x-ray flux over the normal Si(111) monochromator. This flux increase resulted in better time resolution for time-resolved SAXS protein folding studies. SAXS is the only experimental probe which can give overall size and shape indication of the conformational state of a protein. Time-resolved SAXS studies on the refolding of lysozyme was performed (manuscript in preparation). We have performed preliminary experiments using SAXS to study protein folding initiated with a continuous flow mixer. Control studies show that reasonable radius of gyration measurements can be obtained with this method.