HIGH RESOLUTION EM FOR STRUCTURAL BIOLOGY

适用于结构生物学的高分辨率电子显微镜

• 批准号: 6501284
• 负责人: UEL J MCMAHAN
• 金额: $ 196.43万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2004-06-01
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6501284
• 关键词: biomedical equipment purchase; structural biology; transmission electron microscopy

DESCRIPTION (provided by applicant): This is to request funding for a FEI Tecnai G2 F30 High Resolution Transmission Electron Microscope for Structural Biology equipped for tomography and single particle analysis. Tomography provides the highest spatial resolution available for studying cellular organelles in situ or in vitro, while single particle analysis provides the best resolution for studying isolated purified molecules that can not be crystallized. Detailed structural knowledge of organelles and molecules is essential for understanding mechanisms of diseases and developing treatments for them. The microscope would initially support the NIH sponsored research of three well-established investigators at Stanford University School of Medicine: U.J. McMahan, Roger D. Komberg and Stephen J Smith. All are using tomography or single particle analysis in their investigations. With its accompanying equipment, there is no better electron microscope available for the various intended uses of the investigators. These range from determining the structure of molecules at near-atomic resolution (RDK) to characterizing the macromolecular architecture and function of certain organelles crucial for impulse transmission at synapses in the nervous system (UJM) to tracking specific proteins during different stages of synapse formation in order understand their role in that process (SJS). There are currently no other investigators in the broad biomedical research program at Stanford conducting research relying on use of the microscope. However its availability and the usefulness of tomography/single particle analysis would be advertised through a website listing all core facilities and resources at the Medical School and through a lecture/techniques course taught by UJM, RDK, and SJS. Users would be able to schedule time on the microscope via a website as done for other comparable resources at Stanford. The microscope would be generally maintained by a career electron microscope specialist currently in the PI's lab, R.M. Marshall, who has operated microscopes similar to the one proposed. He would also train new users how to operate it, or he would operate it for them. A programmer would be employed part time to maintain and upgrade the software. The maintenance would be financed by an annual operating budget administered through the PT's department by a "service center", as done for other Stanford facilities and resources. The service center would bill users monthly according to their level of usage. This would be the first electron microscope of its type at Stanford.

描述（由申请人提供）：这是为了申请资助FEI Tecnai G2 F30高分辨率透射电子显微镜，用于结构生物学，配备断层扫描和单粒子分析。断层扫描提供了最高的空间分辨率，可用于研究细胞器在原位或体外，而单粒子分析提供了最好的分辨率，研究分离纯化的分子，不能结晶。细胞器和分子的详细结构知识对于理解疾病机制和开发治疗方法至关重要。显微镜最初将支持NIH赞助的斯坦福大学医学院三位知名研究人员的研究：Komberg和Stephen J Smith。所有人都在调查中使用断层扫描或单粒子分析。连同其附带的设备，没有更好的电子显微镜可用于研究人员的各种预期用途。这些范围从以近原子分辨率（RDK）确定分子结构，到表征对神经系统突触冲动传递至关重要的某些细胞器的大分子结构和功能（UJM），再到在突触形成的不同阶段追踪特定蛋白质，以了解它们在该过程中的作用（SJS）。目前，在斯坦福大学的生物医学研究项目中，还没有其他研究人员依靠显微镜进行研究。然而，它的可用性和层析成像/单粒子分析的有用性将通过一个网站，列出所有的核心设施和资源在医学院和通过讲座/技术课程由UJM，RDK，和SJS教授广告。用户可以通过一个网站安排使用显微镜的时间，就像斯坦福大学的其他类似资源一样。显微镜通常由PI实验室的职业电子显微镜专家R.M.维护。马歇尔说，他操作的显微镜与所提出的类似。他还将培训新用户如何操作它，或者他将为他们操作它。将雇用一名程序员兼职维护和升级软件。维护费用将由PT部门的“服务中心”管理的年度运营预算提供，就像斯坦福大学的其他设施和资源一样。服务中心将根据用户的使用水平每月向他们收费。这将是斯坦福大学的第一台电子显微镜。