Procise Protein Sequencing System

描述(申请人提供)：一个由20人组成的用户群 波士顿生物医学研究中心的首席研究员和他们的研究人员 该研究所正在进行20多个由NIH资助的项目，正在申请资金 用于从应用公司购买Procise蛋白质测序系统 生物系统。这些调查人员正在进行广泛的研究 项目，这些项目侧重于蛋白质-蛋白质相互作用和 蛋白质在收缩系统和热中的结构-功能关系 休克蛋白，膜相关能量转导中的亚单位相互作用 和运输系统，细胞死亡的机制和信号通路， 治疗阿尔茨海默病的免疫治疗剂的设计，以及晶体 各种蛋白质的结构。 所要求的仪器将取代已有12年历史的ABI 477，后者具有 最近变得不起作用。需要内部测序设施来满足以下要求 重组DNA产生的蛋白质和蛋白质片段的验证 蛋白质片断的结晶学技术及其评价 学习。N-末端测序与质谱学结合也是 对于涉及识别结合伙伴的研究是必不可少的， 表位的测定、翻译后修饰的鉴定 蛋白质内或蛋白质之间的邻近关系的测定。 所要求的仪器将放置在研究所的蛋白质/多肽核心中 设施，将由核心主任Renne Lu博士监督 设施。陆博士和蔡明仁女士将是指定的操作员和 负责仪器的维护保养。预计 一带一路的研究活动将使这一工具得到很好的利用。然而， 来自其他研究机构的科学家也将获得这一信息 在可获得性基础上的仪器。

DESCRIPTION (provided by applicant): A user group consisting of twenty principal investigators and their research staff at Boston Biomedical Research Institute, working on more than twenty NIH-funded projects, is requesting funds for the purchase of a Procise Protein Sequencing System from Applied Biosystems. These investigators are conducting a wide range of research projects, which focus on topics such as protein-protein interactions and structure-function relationships of proteins in contractile systems and heat shock proteins, subunit interactions in membrane-associated energy transduction and transport systems, mechanisms of cell death and signaling pathways, the design of immunotherapeutic agents to treat Alzheimer disease, and the crystal structures of various proteins. The requested instrument is to replace a 12-year old ABI 477, which has recently become nonfunctional. An in-house sequencing facility is needed for the verification of protein and protein fragments generated by recombinant DNA techniques and the assessment of protein fragments for crystallographic studies. N-terminal sequencing in conjunction with mass spectrometry is also essential for studies involving identification of binding partners, determination of epitopes, identification of post-translational modification sites and determination of proximity relationships within or between proteins. The requested instrument will be housed in the Institute's Protein/Peptide Core Facility and will be supervised by Dr. Renne Lu, the director of the Core Facility. Dr. Lu and Ms. Ming-Jen Tsay will be the designated operators and responsible for the maintenance of the instrument. It is anticipated that research activities at BBRI will keep the instrument well utilized. However, scientists from other research institutions will also be given access to this instrument on an availability basis.