CORE--BIOPHYSICAL AND BIOCHEMICAL FACILITIES

描述： 这个核心将由卢博士领导的蛋白质化学设施组成，并 沃尔特领导下的分析性超速离心机。F·斯塔福德，博士和 胜德下的电子显微镜和免疫细胞化学设备 Mabuchi博士 核心的蛋白质化学部分将为所有项目提供服务 在合成多肽和分析氨基酸的多肽/蛋白质中 组合物和/或序列。它还将提供有关以下方面的建议 多肽/蛋白质的纯化和鉴定。 核心的分析性超速离心部分将主要支持 项目1、2-A、2-B和3。这项服务既提供沉积速度 和沉积平衡分析，以及相关的水动力 分析。 核心B的第三部分将结合使用 电子显微镜和免疫细胞化学。它提供技术专业知识和 关于蛋白质的EM可视化的建议，特别是在改进了 由PI开发的旋转阴影技术，并支持准备 多克隆和单克隆抗体，包括实验室的处理 动物。

Description: This core will consist of a Protein Chemistry facility under Dr. Lu, an Analytical Ultracentrifugation facility under Walter. F. Stafford, Ph.D., and an Electron Microscopy and Immunocytochemistry facility under Katsuhide Mabuchi, Ph.D. The Protein Chemistry part of the Core will provide services for all projects in making synthetic peptides and analyzing peptides/proteins for amino acid compositions and/or sequences. It will also provide advice concerning peptides/proteins purification and characterization. The Analytical Ultracentrifugation part of the Core will primarily support Projects 1, 2-A, 2-B and 3. This service provides both sedimentation velocity and sedimentation equilibrium analysis, as well as related hydrodynamic analyses. The third part of Core B will provide services with the combined use of electron microscopy and immunocytochemistry. It offers technical expertise and advice for the EM visualization of proteins, especially with an improved rotary shadowing technique developed by the PI, and supports the preparation of polyclonal and monoclonal antibodies, including the handling of laboratory animals.