Confocal Microscope

俄勒冈健康科学大学神经科学研究所（NSI）申请激光扫描共聚焦显微镜系统，以支持众多NIH同行评审拨款。选择的供应商是蔡司，其最新技术，激光扫描显微镜-510与非线性光学（LSM-510 NLO），是一个国家的最先进的仪器，允许同时多光子和单光子激发生物组织的最高灵敏度的亚细胞成像和分析解剖结构和生理过程。NSI将以建立仪器核心设施、成本分担、培训以及终身维护和服务合同的方式提供实质性支持。拥有免疫组织化学、荧光显微镜、神经解剖学和体外神经生理学专业知识的5名“主要用户”将使用该仪器75%的时间。仪器时间的平衡将由NSI的8名额外教员占用。总的来说，该仪器将用于支持来自9个不同的NIH研究所的19个活跃的NIH同行评审奖。NSI是一个新成立的小组，由22名来自世界各地学术中心的高级研究人员组成，位于俄勒冈健康科学大学西校区一个全新的（2001年2月），占地66000平方英尺，最先进的建筑。蔡司LSM-510 NLO的增加将提供给亚细胞过程的功能显微镜的新组装组的基本能力。

A laser scanning confocal microscope system is requested for the Neurological Sciences Institute (NSI) of Oregon Health Sciences University in support of numerous NIH peer-reviewed grants. The vendor selected is Zeiss, whose latest technology, the Laser Scanning Microscope-510 with Non Linear Optics (LSM-510 NLO), is a state-of- the-art instrument that allows simultaneous multi-photon and single- photon excitation of biological tissue for the highest-sensitivity subcellular imaging and analysis of anatomical structure and physiological processes. The NSI will provide substantial support in the way of establishing a central core-facility for the instrument, cost- sharing, training, and a life-time maintenance and service contract. A group of 5 "major users" with expertise in immunohistochemistry, fluorescence microscopy, neuroanatomy, and in vitro neurophysiology will employ 75% of the instrument's time. The balance of the instrument's time will be occupied by 8 additional faculty of the NSI. Altogether, the instrument will be used in support of 19 active NIH peer- reviewed awards from 9 different NIH institutes. The NSI is a newly formed group of 22 senior investigators that have been recruited from academic centers throughout the world that is housed in a brand new (February, 2001), 66,000 square foot, state-of-the-art building on the West Campus of Oregon Health Science University. The addition of a Zeiss LSM-510 NLO will make available to the newly assembled group essential capabilities in functional microscopy of subcellular processes.