GENOMIC ANALYSIS OF LAMBDOID, TB & OTHER BACTERIOPHAGES

LAMBDOID，TB 的基因组分析

• 批准号: 6386103
• 负责人: ROGER W HENDRIX
• 金额: $ 22.52万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-01-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6386103
• 关键词: bacterial virus; bacteriophage lambda; biochemical evolution; computer assisted sequence analysis; genetic polymorphism; nucleic acid sequence; technology /technique development; virus DNA; virus genetics

The dsDNA bacteriophages are the most numerous taxon of evolving organisms in the biosphere. However, their classification remains unclear and their inter-relationships are poorly understood. Characterization of these dsDNA phages by whole genome sequencing provide more detailed information of these relationships and provides an emerging picture in which essentially all dsDNA tailed bacteriophages have shared ancestry, even through they infect different hosts and have varied viral morphologies. Comparison of phage genomes also shows that they are constructed from a series of genetic modules that are joined into functional groups and they are essentially mosaic in nature. However, it is not known how many different modular segments there are, or how many variants exist of each module. Moreover, the mechanisms by which exchange occurs have yet to be clearly elucidated. We propose to investigate the process of phage evolution through the sequencing of phage genomes and comparisons of sequences, genome organization and function relationships. In addition, we propose to survey unselected phage populations in order to obtain a broader view of the diversity of bacteriophages that naturally exist in the environment. More sophisticated software systems will be developed that will greatly simplify the data analysis steps and provide automated methods for genome comparisons. These studies will not only lead to new insights into the mechanisms of viral evolution but will provide a valuable resource for other researchers, including those studying basic phage biology such as DNA replication, gene expression and macromolecular assembly as well as those interested in the application of phages to clinical problems, such as diagnosis and phage therapy.

双链 DNA 噬菌体是生物圈中数量最多的进化生物体分类单元。然而，它们的分类仍然不清楚，而且它们之间的相互关系也知之甚少。通过全基因组测序对这些 dsDNA 噬菌体进行表征，提供了有关这些关系的更详细信息，并提供了一幅新的图景，其中基本上所有 dsDNA 尾噬菌体都具有共同的祖先，即使它们感染不同的宿主并具有不同的病毒形态。噬菌体基因组的比较还表明，它们是由一系列连接成功能组的遗传模块构建的，本质上是镶嵌的。然而，尚不清楚有多少个不同的模块部分，或者每个模块存在多少种变体。此外，交换发生的机制尚未明确阐明。我们建议通过噬菌体基因组测序以及序列比较、基因组组织和功能关系来研究噬菌体进化过程。此外，我们建议调查未选择的噬菌体种群，以便更广泛地了解环境中自然存在的噬菌体的多样性。将开发更复杂的软件系统，这将大大简化数据分析步骤并提供基因组比较的自动化方法。这些研究不仅将带来对病毒进化机制的新见解，而且将为其他研究人员提供宝贵的资源，包括那些研究基本噬菌体生物学（例如DNA复制、基因表达和大分子组装）的研究人员以及那些对噬菌体应用于临床问题（例如诊断和噬菌体治疗）感兴趣的研究人员。