CORE--RADIATION MEDICINE

核心--放射医学

• 批准号: 6501423
• 负责人: Francisco S Pardo
• 金额: $ 18.37万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-21 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6501423
• 关键词: DNA damage; biomedical facility; cell growth regulation; ionizing radiation; laboratory mouse; laboratory rat; mathematical model; model design /development; neoplasm /cancer; neoplasm /cancer radiation therapy; nonhuman therapy evaluation; radiation genetics; radiation sensitivity

The Radiation Medicine Shared Resource provides the opportunity for UCSD Cancer Center investigators to pursue an interactive approach to the determination of intrinsic radiation sensitivity. The conduct of radiation assays quantifying radiation sensitivity to ionizing radiation in vitro, as well as mathematical modeling of survival parameters, is the cornerstone of services provided. As a corollary to this purpose, the Facility lends expertise to researchers studying the cell cycle control of DNA damage and genomic instability induced by ionizing radiation both in vitro and in vivo. Services include: 1. Irradiation of Cells, Cell Lines, and Feeder Cells; Clonogenic Radiation Survival Studies. Cell strains and cell lines are irradiated with a variety of radiation fraction sizes, for single fraction experiments, and at a variety of infraction intervals, for multiple fraction experiments. Similarly, cells are irradiated at high dose per fraction for experiments requiring lethally irradiated feeder cells. Nearby Resource cell culture and incubator equipment provide for relative ease of conducting combined radiation sensitization/protection, chemosensitivity, and gene therapy approaches. 2. Whole or Partial Body Irradiation of Mice or Rats. Whole body irradiation of mouse or rat species are irradiated for a variety of purposes ranging from single fraction whole body irradiation for immunosuppression to fractionated tumor control, tumor induction, or normal body irradiation for immunosuppression to fractionated tumor control, tumor induction, or normal tissue tolerance studies. For experimental protocols requiring the irradiation of specific body regions, custom lead shielding has been tailored to the treatment volume while sparing nearby normal tissues. 3. Assistance with Radiobiologic Data Interpretation, Survival Modeling, and Illustration. Recommendations on the optimal method of interpreting radiation cell survival data allow investigators familiarity with multiple cell survival models and the derivation of interrelated radiation cell survival parameters to be used in subsequent statistical analysis.

放射医学共享资源为加州大学圣地亚哥分校癌症中心的研究人员提供了一个机会，以追求一种互动的方法来确定固有的辐射敏感性。进行辐射分析，量化对体外电离辐射的辐射敏感性，以及建立生存参数的数学模型，是所提供服务的基础。作为这一目的的必然结果，该设施为研究人员在体外和体内电离辐射诱导的DNA损伤和基因组不稳定性的细胞周期控制提供专业知识。业务包括：1；细胞、细胞系和饲养细胞的辐照克隆性辐射生存研究。细胞株和细胞系在单组分实验中用各种辐射分数大小照射，在多组分实验中用各种违规间隔照射。同样，在实验中，细胞按每部分的高剂量辐照，需要辐照致死的饲养细胞。附近的资源细胞培养和培养设备提供了相对容易进行联合辐射敏化/保护，化学敏感性和基因治疗方法。2. 小鼠或大鼠全身或局部照射。小鼠或大鼠的全身照射有多种用途，从用于免疫抑制的单次全身照射到分级肿瘤控制、肿瘤诱导，或用于免疫抑制的正常全身照射到分级肿瘤控制、肿瘤诱导或正常组织耐受研究。对于需要照射特定身体区域的实验方案，定制的铅屏蔽已根据治疗量量身定制，同时保留附近的正常组织。3. 协助放射生物学数据解释，生存建模和说明。关于解释辐射细胞存活数据的最佳方法的建议使研究人员熟悉多种细胞存活模型和相关辐射细胞存活参数的推导，以便在随后的统计分析中使用。