CORE--RADIATION MEDICINE

核心--放射医学

• 批准号: 6599280
• 负责人: Francisco S Pardo
• 金额: $ 18.37万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-11 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6599280
• 关键词: DNA damage; biomedical facility; cell growth regulation; ionizing radiation; laboratory mouse; laboratory rat; mathematical model; model design /development; neoplasm /cancer; neoplasm /cancer radiation therapy; nonhuman therapy evaluation; radiation genetics; radiation sensitivity

The Radiation Medicine Shared Resource provides the opportunity for UCSD Cancer Center investigators to pursue an interactive approach to the determination of intrinsic radiation sensitivity. The conduct of radiation assays quantifying radiation sensitivity to ionizing radiation in vitro, as well as mathematical modeling of survival parameters, is the cornerstone of services provided. As a corollary to this purpose, the Facility lends expertise to researchers studying the cell cycle control of DNA damage and genomic instability induced by ionizing radiation both in vitro and in vivo. Services include: 1. Irradiation of Cells, Cell Lines, and Feeder Cells; Clonogenic Radiation Survival Studies. Cell strains and cell lines are irradiated with a variety of radiation fraction sizes, for single fraction experiments, and at a variety of infraction intervals, for multiple fraction experiments. Similarly, cells are irradiated at high dose per fraction for experiments requiring lethally irradiated feeder cells. Nearby Resource cell culture and incubator equipment provide for relative ease of conducting combined radiation sensitization/protection, chemosensitivity, and gene therapy approaches. 2. Whole or Partial Body Irradiation of Mice or Rats. Whole body irradiation of mouse or rat species are irradiated for a variety of purposes ranging from single fraction whole body irradiation for immunosuppression to fractionated tumor control, tumor induction, or normal body irradiation for immunosuppression to fractionated tumor control, tumor induction, or normal tissue tolerance studies. For experimental protocols requiring the irradiation of specific body regions, custom lead shielding has been tailored to the treatment volume while sparing nearby normal tissues. 3. Assistance with Radiobiologic Data Interpretation, Survival Modeling, and Illustration. Recommendations on the optimal method of interpreting radiation cell survival data allow investigators familiarity with multiple cell survival models and the derivation of interrelated radiation cell survival parameters to be used in subsequent statistical analysis.

放射医学共享资源为加州大学圣地亚哥分校癌症中心的研究人员提供了采用交互式方法来确定固有辐射敏感性的机会。进行辐射测定，量化体外电离辐射的辐射敏感性，以及生存参数的数学模型，是所提供服务的基石。作为这一目的的必然结果，该设施为研究人员提供专业知识，研究由体外和体内电离辐射引起的 DNA 损伤和基因组不稳定性的细胞周期控制。服务包括： 1. 细胞、细胞系和饲养细胞的辐照；克隆形成辐射生存研究。对于单部分实验，细胞株和细胞系用各种辐射分数大小进行照射；对于多分数实验，以各种损伤间隔进行照射。类似地，对于需要致命照射的饲养细胞的实验，每个部分的细胞都受到高剂量的照射。附近的资源细胞培养和培养箱设备可以相对轻松地进行组合辐射增敏/保护、化学增敏和基因治疗方法。 2. 小鼠或大鼠的全身或部分身体照射。小鼠或大鼠物种的全身照射可用于多种目的，从用于免疫抑制的单次全身照射，到用于免疫抑制的分段肿瘤控制、肿瘤诱导或正常身体照射，再到分段肿瘤控制、肿瘤诱导或正常组织耐受性研究。对于需要照射特定身体区域的实验方案，定制的铅屏蔽已根据治疗体积进行定制，同时保护附近的正常组织。 3. 协助放射生物学数据解释、生存建模和说明。关于解释辐射细胞存活数据的最佳方法的建议使研究人员能够熟悉多种细胞存活模型，并推导相关辐射细胞存活参数以用于后续统计分析。