MULTIDISCIPLINARY MICRO CT-3D IMAGING FACILITY

多学科微型 CT-3D 成像设备

• 批准号: 6394655
• 负责人: Erik Leo Ritman
• 金额: $ 38.13万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-30 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6394655
• 关键词: bioimaging /biomedical imaging; biomedical automation; biomedical equipment development; biomedical facility; computed axial tomography; computer program /software; human tissue; image processing; information display; kidney function; laboratory rat; radiography; renal tubule; single photon emission computed tomography

DESCRIPTION (Verbatim from Applicant's Abstract): This resubmission continues the overall goal which is to develop and apply an x-ray micro-CT scanner for generating 3-D images, of sufficient spatial resolution and specimen size, to allow quantitation of intact organ microarchitecture in terms of all Basic Functional Units (BFU, e.g., glomerulus and its nephron) as well as the transport mechanisms within the organ. The application is focused on intact rodent organs or similar-sized (1-8 cm3) "biopsies" from large animals and humans. In response to the previous review, more detail of scanner characteristics and how these relate to users of the scanner, are provided. The hypothesis of this proposal is that bench-top x-ray micro-CT can provide important new quantitative information about organ micro architecture by a combination of hardware and software enhancements motivated by their utility in meeting new biomedical research needs, such as defining the phenotype of genetically modified mice. AIM I - expand the hardware and associated software to: (a) extend the capability of our current x-ray detection device with "super"-resolution scanning modes & reconstruction algorithms and with "local" reconstruction algorithms, (b) add helical scanning capability, (c) increase x-ray flux with x-ray optics. AIM II - Image dvnamic processes bv scanning 'snap' frozen specimen: This involves snap-freezing the specimen immediately after (a) the desired distribution of contrast agent or (b) degree of mechanical distortion (e.g., inflation) has been achieved in vivo and keeping it frozen during the scan. AIM III - Quantitation of 3-D microarchitecture and associated 3-D function by image analysis via (a) extension of automated vascular "tree" finding/analysis algorithm; (b) develop a visualization tool for guiding and evaluating the automated image analysis; (c) quantitation of local strains throughout an organ that is mechanically distorted during a scan; and (d) analysis of the fluid dynamic implications of vessel branching geometry. AIM IV - Continue to provide the 3D micro-CT imaging service to investigator-users so that this experience will guide innovation, improvement and/or expansion of the imaging repertoire of the micro-CT methodology.

描述（申请人摘要的逐字记录）：此重新提交仍在继续 总体目标是开发和应用 X 射线微型 CT 扫描仪 生成具有足够空间分辨率和样本尺寸的 3D 图像， 允许对所有基本的完整器官微结构进行定量 功能单位（BFU，例如肾小球及其肾单位）以及 器官内的转运机制。该应用程序专注于完好无损 啮齿动物器官或类似大小（1-8 cm3）的大型动物“活组织检查”以及 人类。为了回应之前的评论，扫描仪的更多细节 提供了特征以及这些特征与扫描仪用户的关系。这 该提案的假设是台式 X 射线显微 CT 可以提供 关于器官微结构的重要新定量信息 硬件和软件增强功能的结合，源于其实用性 满足新的生物医学研究需求，例如定义表型 转基因小鼠。 AIM I - 扩展硬件和相关软件以： (a) 扩展 我们当前的 X 射线检测设备具有“超”分辨率的能力 扫描模式和重建算法以及“局部”重建 算法，(b) 添加螺旋扫描功能，(c) 增加 X 射线通量 X 射线光学。 AIM II - 通过扫描“快照”冷冻标本进行图像动态处理：这 包括在 (a) 所需的时间后立即速冻样本 造影剂的分布或 (b) 机械变形程度（例如， 膨胀）已在体内实现并在扫描过程中保持冷冻。 AIM III - 3-D 微结构和相关 3-D 功能的定量 通过 (a) 扩展自动血管“树”查找/分析进行图像分析 算法; (b) 开发可视化工具来指导和评估 自动图像分析； (c) 整个器官局部应变的定量 扫描过程中机械变形； (d) 流体分析 血管分支几何的动态影响。 AIM IV - 继续提供3D显微CT成像服务 研究者-用户，以便这种经验能够指导创新、改进 和/或扩展显微 CT 方法的成像库。