Central CART Peptide and Serotonin in Feeding Behavior

中枢 CART 肽和血清素在进食行为中的作用

• 批准号: 6340100
• 负责人: SUSAN M AJA
• 金额: $ 4.02万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-10-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6340100
• 关键词: amphetamines; cocaine; eating; gene induction /repression; high performance liquid chromatography; immunocytochemistry; laboratory rat; neurogenetics; protooncogene; psychomotor function; psychopharmacology; rhombencephalon; satiations; serotonin

DESCRIPTION: The proposed research will explore potential roles of serotonin (5-HT) in the reduction of rat feeding behavior by cocaine- and amphetamine-regulated transcript (CART). Preliminary work from this laboratory extends reports of anorectic responses to intracerebroventricularly administered CART peptides, showing that CART reduces licking behavior, produces serotonin syndrome-like motor signs, and likely exerts these actions at tissues surrounding the fourth ventricle (4V). The specific aims of this proposal are to assess the role of 5-HT in effects of 4V CART on liquid food intake and ingestion patterns, postural motor function, and genomic expression. The strategy will be to abolish 5-HT neurotransmission by selectively lesioning hindbrain serotonergic neurons, or blocking activity at 5-HT2C receptors, and then to examine the effects of 4V CART on intake of a nutritionally complete liquid diet under ad libitum and food deprivation feeding paradigms. Examining mechanisms underlying anorectic and other behavioral actions of CART will contribute to a more complete understanding of the controls of food intake, and may aid in the evaluation of CART as a treatment for obesity.

产品说明：本研究将探讨5-羟色胺（5-HT）在可卡因和苯丙胺调节转录物（CART）降低大鼠摄食行为中的潜在作用。该实验室的初步工作扩展了对脑室内给予CART肽的厌食反应的报告，表明CART减少了舔行为，产生5-羟色胺综合征样运动体征，并可能在第四脑室（4V）周围的组织中发挥这些作用。该提案的具体目的是评估5-HT在4V CART对液体食物摄入和摄取模式、姿势运动功能和基因组表达的影响中的作用。该策略将是通过选择性损伤后脑多巴胺能神经元或阻断5-HT 2C受体的活性来消除5-HT神经传递，然后检查4V CART对在随意和食物剥夺喂养模式下摄入营养完全的液体饮食的影响。研究CART的厌食和其他行为作用的机制将有助于更全面地了解食物摄入的控制，并可能有助于评估CART作为肥胖治疗的作用。