THE ROLE OF INHIBITION IN THE AUDITORY BRAINSTEM

该项目将研究栖息在家鸡成熟和发育中的脑干听觉系统中的声学处理中的作用。这一实验包括三个方面，构成了具体目标：1)评估GABA能输入对大细胞核内突触前和突触后成分的影响；2)评估初级兴奋性和抑制性输入对上橄榄核神经元加工的相对贡献；3)描述和调查抑制性输入对大细胞核和板层核发育过程中抑制性输入的有效性和时间特性的影响。目的1在体内使用多管电极对不同GABA受体电极的贡献进行药理学分析，从而对不同GABA受体亚型的贡献进行药理学分析，这些亚型在大细胞核突触前和突触后终末有不同的表达。AIMS 2和AIMS 3将在体外制备自溶素，这将允许从实验上将输入分离到脑干核团，并提供获得神经元生物物理性质的途径。这些研究将有助于更全面地了解脊椎动物神经系统中的声处理。此外，发育研究可能揭示可能对聋人功能恢复起作用的因素。了解发育对突触特性的影响可能有助于为生命早期被剥夺声刺激的患者开发适当的治疗方法。

The project will investigate the role of inhabitation in acoustic processing in the mature and developing brainstem auditory system of the domestic chicken. This stay involves a three-pronged approach that constitute the specific aims: 1) to asses the influence of GABAergic input on both pre and postsynaptic elements in thee nucleus in the nucleus magnocellularis; 2) to asses the relative contribution of the primary excitatory and inhibitory inputs to processing of neurons in the superior olivary nucleus; 3) to describe and investigate influences on the efficacy and temporal characteristics of inhibitory inputs to nucleus magnocellularis and nucleus laminar is during development. Aim 1 will be addressed in vivo using multibarrel electrodes which allow for the pharmacological analysis of the contribution of various GABA receptor electrodes which allow for the pharmacological analysis of the contribution of various GABA receptor subtypes which are differently expressed in pre and postsynaptic terminals in nucleus magnocellularis. Aims 2 and 3 will autolysin in vitro preparation that will allow for the experimental segregation of inputs to brainstem nuclei and provide access to the biophysical properties of the neurons. These studies will contribute to a more complete understanding of acoustic processing in vertebrate nervous systems. Furthmore, developmental studies may reveal factors that could play a role in recovery of function in deaf patients. Understanding developmental influences on synaptic properties may contribute to the development of appropriate therapies for patients deprived of acoustic stimulation early in life.