THE ROLE OF INHIBITION IN THE AUDITORY BRAINSTEM

抑制在听觉脑干中的作用

• 批准号: 6626849
• 负责人: R. Michael Burger
• 金额: $ 4.64万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-04 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6626849
• 关键词: brain mapping; brain stem; chickens; developmental neurobiology; electrophysiology; gamma aminobutyrate; neural information processing; neural inhibition; neuroanatomy; neurons; postdoctoral investigator; sound perception; synapses

The project will investigate the role of inhabitation in acoustic processing in the mature and developing brainstem auditory system of the domestic chicken. This stay involves a three-pronged approach that constitute the specific aims: 1) to asses the influence of GABAergic input on both pre and postsynaptic elements in thee nucleus in the nucleus magnocellularis; 2) to asses the relative contribution of the primary excitatory and inhibitory inputs to processing of neurons in the superior olivary nucleus; 3) to describe and investigate influences on the efficacy and temporal characteristics of inhibitory inputs to nucleus magnocellularis and nucleus laminar is during development. Aim 1 will be addressed in vivo using multibarrel electrodes which allow for the pharmacological analysis of the contribution of various GABA receptor electrodes which allow for the pharmacological analysis of the contribution of various GABA receptor subtypes which are differently expressed in pre and postsynaptic terminals in nucleus magnocellularis. Aims 2 and 3 will autolysin in vitro preparation that will allow for the experimental segregation of inputs to brainstem nuclei and provide access to the biophysical properties of the neurons. These studies will contribute to a more complete understanding of acoustic processing in vertebrate nervous systems. Furthmore, developmental studies may reveal factors that could play a role in recovery of function in deaf patients. Understanding developmental influences on synaptic properties may contribute to the development of appropriate therapies for patients deprived of acoustic stimulation early in life.

本研究将探讨栖息地在家鸡成熟发育的脑干听觉系统中的作用。本研究从三个方面探讨GABA能输入对大细胞核内突触前和突触后成分的影响：（1）评价初级兴奋性和抑制性输入对上级橄榄核神经元加工的相对贡献;（3）描述和探讨发育过程中对大细胞核和板层核抑制性输入的效应和时间特征的影响。目的1将在体内使用多管电极来解决，该多管电极允许对各种GABA受体电极的贡献进行药理学分析，这些电极允许对在大细胞核中的突触前和突触后末端中不同表达的各种GABA受体亚型的贡献进行药理学分析。目的2和3将自溶素在体外制备，这将允许输入脑干核的实验分离，并提供对神经元的生物物理性质的访问。这些研究将有助于更全面地了解脊椎动物神经系统中的声处理。此外，发展研究可能揭示可能在耳聋患者功能恢复中起作用的因素。了解发育对突触特性的影响可能有助于为生命早期被剥夺声刺激的患者制定适当的治疗方法。