ORAL IMMUNIZATION WITH GINGIPAIN DELIVERED BY SALMONELLA
ORAL IMMUNIZATION WITH GINGIPAIN DELIVERED BY SALMONELLA
批准号:
6350576
负责人:
FRANK C GIBSON
金额:
$4.73万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2001
资助国家:
美国
项目状态:
未结题
起止时间:
2001-02-01 至
关键词:
Bacteroides gingivalis Salmonella typhimurium antigen antibody reaction attenuated microorganism bacterial vaccines biological models biotherapeutic agent chimeric proteins cysteine endopeptidases host organism interaction immunization immunoglobulin A immunoglobulin G immunoglobulin M laboratory mouse microorganism metabolism mucosal immunity oral administration periodontitis periodontium disorder vaccine development
中文摘要
牙龈卟啉单胞菌是成人牙周病的重要致病菌。一些研究表明,由该生物体产生的半胱氨酸蛋白酶(牙龈菌蛋白酶)在牙龈卟啉单胞菌诱导的牙周病中起重要作用。 最近,一些研究表明,用牙龈卟啉菌蛋白酶或这些牙龈卟啉菌蛋白酶的区域免疫小鼠提供了对随后的牙龈卟啉单胞菌攻击的保护。由于牙龈卟啉单胞菌感染开始于牙周袋,我们认为牙龈卟啉单胞菌蛋白酶递送至粘膜表面代表了尝试预防牙龈卟啉单胞菌诱导的疾病的新方法。减毒鼠伤寒沙门氏菌口服免疫已被证明是一种有效的方式,以提高在粘膜表面的宿主免疫应答。我们建议构造衰减S。表达牙龈菌蛋白酶区域特异性/LT-B(E.大肠杆菌不耐热肠毒素)融合体。将在小鼠-牙龈卟啉单胞菌口腔感染模型中评价这些沙门氏菌菌株的口服免疫保护免受牙龈卟啉单胞菌介导的骨丢失的能力。这些数据将提供深入了解粘膜免疫策略的有用性,以防止牙龈卟啉单胞菌诱导的疾病,并开始的过程中,牙龈卟啉菌蛋白酶特异性表位介导这种保护性的宿主免疫应答。
英文摘要
Porphyromonas gingivalis is an important pathogen in adult periodontal disease. Several studies have suggested that the cysteine-proteases (gingipains) produced by this organism play an important role during P. gingivalis-induced periodontal disease. Recently, several investigations indicate that immunization of mice with gingipains or regions of these gingipains provide protection from subsequent P. gingivalis challenge. As, P. gingivalis infection begins in the periodontal pocket, we believe that delivery of gingipains to mucosal surfaces represents a novel approach to attempt to prevent P. gingivalis-induced disease. Oral immunization with attenuated Salmonella typhimurium has been demonstrated to be an effective way to enhance the host immune response at mucosal surfaces. We propose to construct attenuated S. typhimurium strains expressing gingipain region-specific/LT-B (binding domain of E. coli heat-labile enterotoxin) fusions. The ability of oral immunization of these Salmonella strains to protect against P. gingivalis-mediated bone loss will be evaluated m a mouse-P. gingivalis oral infection model. These data will provide insight into the usefulness of mucosal immunization strategies to prevent P. gingivalis-induced disease and begin the process of characterizing which gingipain-specific epitopes mediate this protective host immune response.
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会议论文
PPARs and periodontal disease
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批准号:8968210
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项目类别:
-
资助金额:$26.1万
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财政年份:2015
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负责人:FRANK C GIBSON
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依托单位:
PPARs and periodontal disease
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批准号:9309421
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项目类别:
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资助金额:$17.28万
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财政年份:2015
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负责人:FRANK C GIBSON
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依托单位:
Oral Macrophage Function in the Context of Periodontal Disease and HIV Infection
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批准号:8739537
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项目类别:
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资助金额:$62.14万
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财政年份:2013
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负责人:FRANK C GIBSON
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依托单位:
Oral Macrophage Function in the Context of Periodontal Disease and HIV Infection
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批准号:8730755
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项目类别:
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资助金额:$48.8万
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财政年份:2013
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负责人:FRANK C GIBSON
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依托单位:
Interferon Regulatory Factors and Periodontal Disease
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批准号:8287188
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项目类别:
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资助金额:$21.13万
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财政年份:2011
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负责人:FRANK C GIBSON
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依托单位:
Interferon Regulatory Factors and Periodontal Disease
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批准号:8190148
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项目类别:
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资助金额:$25.35万
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财政年份:2011
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负责人:FRANK C GIBSON
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依托单位:
Innate Immunity, Lipid Signaling, and Chronic Infection
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批准号:7790038
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项目类别:
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资助金额:$25.76万
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财政年份:2010
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负责人:FRANK C GIBSON
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依托单位:
IInfection-Elicited Oral Bone Loss: TLR2, Ontogency, and Porphromonas Gingivalis
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批准号:7781398
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项目类别:
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资助金额:$31.53万
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财政年份:2007
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负责人:FRANK C GIBSON
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依托单位:
IInfection-Elicited Oral Bone Loss: TLR2, Ontogency, and Porphromonas Gingivalis
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批准号:8125507
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项目类别:
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资助金额:$5.91万
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财政年份:2007
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负责人:FRANK C GIBSON
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依托单位:
IInfection-Elicited Oral Bone Loss: TLR2, Ontogency, and Porphromonas Gingivalis
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批准号:7278527
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项目类别:
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资助金额:$31.81万
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财政年份:2007
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负责人:FRANK C GIBSON
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依托单位:
IInfection-Elicited Oral Bone Loss: TLR2, Ontogency, and Porphromonas Gingivalis
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批准号:7383108
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项目类别:
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资助金额:$31.85万
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财政年份:2007
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负责人:FRANK C GIBSON
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依托单位:
IInfection-Elicited Oral Bone Loss: TLR2, Ontogency, and Porphromonas Gingivalis
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批准号:7579128
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项目类别:
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资助金额:$31.85万
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财政年份:2007
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负责人:FRANK C GIBSON
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依托单位:
P. gingivalis Capsule in Cell Inflammation
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批准号:6613063
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项目类别:
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资助金额:$28.18万
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财政年份:2003
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负责人:FRANK C GIBSON
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依托单位:
The Role of p. gingivalis Capsule in Cell Inflammation
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批准号:6846639
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项目类别:
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资助金额:$24.15万
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财政年份:2003
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负责人:FRANK C GIBSON
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依托单位:
P. gingivalis Capsule in Cell Inflammation
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批准号:7169840
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项目类别:
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资助金额:$22.9万
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财政年份:2003
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负责人:FRANK C GIBSON
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依托单位:
The Role of p. gingivalis Capsule in Cell Inflammation
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批准号:6740925
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项目类别:
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资助金额:$28.18万
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财政年份:2003
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负责人:FRANK C GIBSON
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依托单位:
The Role of p. gingivalis Capsule in Cell Inflammation
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批准号:7010327
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项目类别:
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资助金额:$23.58万
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财政年份:2003
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负责人:FRANK C GIBSON
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依托单位:
ORAL IMMUNIZATION WITH GINGIPAIN DELIVERED BY SALMONELLA
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批准号:6070128
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项目类别:
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资助金额:$4.26万
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财政年份:2000
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负责人:FRANK C GIBSON
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依托单位:
Innate Immunity, Lipid Signaling, and Chronic Infection
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批准号:8527673
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项目类别:
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资助金额:$22.87万
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财政年份:--
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负责人:FRANK C GIBSON
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依托单位:
Innate Immunity, Lipid Signaling, and Chronic Infection
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批准号:8380357
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项目类别:
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资助金额:$29.39万
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财政年份:--
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负责人:FRANK C GIBSON
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依托单位:
海外基金