The impact of ER stress on Salmonella Typhimurium infections
内质网应激对鼠伤寒沙门氏菌感染的影响
基本信息
- 批准号:10708073
- 负责人:
- 金额:$ 38.1万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-09-20 至 2027-07-31
- 项目状态:未结题
- 来源:
- 关键词:ATF6 geneActinsApoptosisApoptoticAutophagocytosisBacterial InfectionsBindingBiological ProcessCalciumCalcium SignalingCell Death InductionCell physiologyCellsColitisComplexCytoskeletonDataDevelopmentEndoplasmic ReticulumEpithelial CellsFunctional disorderFutureGastroenteritisGastrointestinal tract structureGene ExpressionGene TargetingGenesGram-Negative BacteriaHomeostasisHumanImmune responseImmune signalingImmunologic ReceptorsIn VitroInduction of ApoptosisInfectionInflammationInflammatoryInflammatory Bowel DiseasesInflammatory ResponseInterventionIntestinesLigandsLinkLipidsMediatingMembraneModelingMusMutant Strains MiceMutationNutrientPathogenesisPathologyPathway interactionsPattern recognition receptorPeptidoglycanPlayPopulationPredispositionProcessProtein InhibitionProteinsRegulationResistanceResourcesRoleSalmonellaSalmonella infectionsSalmonella typhimuriumSignal TransductionSourceStreptomycinTestingTherapeuticTranslationsWorkXBP1 genebiological adaptation to stressconditional mutantdextran sulfate sodium induced colitisendoplasmic reticulum stressexperimental studygut inflammationinterestintestinal epitheliummicrobiotamonocytepathogenprotein complexprotein misfoldingreceptorrelease of sequestered calcium ion into cytoplasmresponsesphingosine 1-phosphatetissue/cell culture
项目摘要
Project Summary
NOD1 and NOD2 are Pattern Recognition Receptors that sense fragments of bacterial peptidoglycans, and are able to detect perturbations in cellular processes such as the regulation of the actin cytoskeleton and disturbance in endoplasmic reticulum (ER) homeostasis. Under different stressful conditions, such as bacterial infections, protein misfolding and perturbations in calcium homeostasis, the ER is unable to maintain homeostasis and activates the unfolded protein response (UPR). Within the UPR three transmembrane receptors, IRE1α, PERK and ATF6, are activated and regulate biological processes such as inhibition of protein translation, autophagy,
and inflammation to reestablish cellular homeostasis. NOD1 and NOD2 have been implicated in ER stress- induced inflammation, by acting downstream of IRE1α in the UPR to induce inflammatory responses. This link between the UPR and NOD1/2 signaling is of particular interest in intestinal inflammation since mutations in genes associated with the UPR (XBP1) and innate immune signaling (NOD2) have been associated with intestinal epithelial cell (IEC) dysfunction in intestinal inflammatory diseases. Salmonella Typhimurium (S. Tm) is a gram-negative bacterium that induces robust inflammation, partially dependent on NOD1/2 activation, of the intestinal epithelium resulting in gastroenteritis. As a survival mechanism, S. Tm has adapted to these
inflammatory conditions in the intestinal tract by utilizing products of inflammation as a nutrient source to outcompete the resident microbiota. Considering the importance of ER stress in intestinal inflammation and the fact that S. Tm is a major cause of gastroenteritis, it is surprising that ER stress in the context of Salmonella infections is significantly underexplored. Furthermore, it is currently unknown whether S. Tm can exploit ER stress-induced inflammation resulting in luminal expansion. Our objectives are to investigate the link between ER stress and inflammation in the S. Tm-induced colitis model. Our central hypothesis is that activation of the ER stress response in the gastrointestinal tract contributes significantly to S. Tm-induced inflammation, luminal expansion and pathology. We will test our hypothesis by pursuing the following three aims. 1) Determine the contribution of calcium flux and UPR activation to NOD1/2 signaling. In in vitro experiments we will determine which branches of the UPR, in conjunction with dysregulation of calcium signaling, contributes to NOD1/2 activation. 2) Determine the role of CHOP in the S. Tm-induced colitis model. Using Chop-/- mice and conditional mutant mice we will investigate the role of CHOP in S. Tm-induced inflammation and outgrowth. 3) Determine the role of NOD1/2 and IRE1α in response to S. Tm. We will investigate the role of IRE1α in Nod1/2-/- mice in the S. Tm-induced colitis model. Characterizing the mechanisms downstream of the UPR that orchestrate ER
stress-induced responses is necessary to safely modulate this process for the development of future therapeutics and will significantly increase our understanding in Salmonella pathogenesis.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Arina Marijke Keestra-Gounder其他文献
Arina Marijke Keestra-Gounder的其他文献
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{{ truncateString('Arina Marijke Keestra-Gounder', 18)}}的其他基金
The impact of ER stress on Salmonella Typhimurium infections
内质网应激对鼠伤寒沙门氏菌感染的影响
- 批准号:
10565316 - 财政年份:2022
- 资助金额:
$ 38.1万 - 项目类别:
NOD2 promotes coxsackievirus entry and pathogenesis
NOD2促进柯萨奇病毒进入和发病机制
- 批准号:
10407068 - 财政年份:2021
- 资助金额:
$ 38.1万 - 项目类别:
NOD2 promotes coxsackievirus entry and pathogenesis
NOD2促进柯萨奇病毒进入和发病机制
- 批准号:
10285845 - 财政年份:2021
- 资助金额:
$ 38.1万 - 项目类别:
Activation of the NOD1 and NOD2 signaling pathways
NOD1 和 NOD2 信号通路的激活
- 批准号:
10041261 - 财政年份:2020
- 资助金额:
$ 38.1万 - 项目类别:
Activation of the NOD1 and NOD2 signaling pathways
NOD1 和 NOD2 信号通路的激活
- 批准号:
10172844 - 财政年份:2020
- 资助金额:
$ 38.1万 - 项目类别:
Targeting ER Stress in Inflammatory Bowel Disease
针对炎症性肠病的内质网应激
- 批准号:
9019324 - 财政年份:2016
- 资助金额:
$ 38.1万 - 项目类别:
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