CORE--MURINE/HEMATOLOGY COAGULATION

核心--鼠/血液学凝固

• 批准号: 6504154
• 负责人: DZUNG LE
• 金额: $ 13.59万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6504154
• 关键词: biomedical facility; blood coagulation; fibrinolysis; hematology; laboratory mouse; liver metabolism; molecular pathology; thrombosis; tissue /cell culture; vascular endothelium

The Murine Hematology/Coagulation Core's primary function is to check for hematological and related metabolic abnormalities in the different mouse strains produced by different Projects. The following tasks will be carried out by this core: 1) Identifying abnormalities in blood cell number and morphology, and abnormalities in hemostasis. Measurements will include: a) Hematological parameters: quantitation of red blood cells, white blood cells (including subsets), and platelets in whole blood. b) Hemostatic parameters: bleeding time, prothrombin time (PT), activated partial thromboplastin time (APTT), and thrombin time. c) Fibrinolytic parameters: dilute whole blood clot lysis time. d) Anticoagulation parameters: protein C, protein S and antithrombin activities. 2) Identifying abnormalities in endothelial cell function with respect to hemostasis. The effect of genetically altered endothelial cell surface glycosaminoglycans (project #5) upon blood coagulation and fibrinolysis will be assessed by use of endothelial cell cultures derived from different tissues of control and transgenic mice. Cell surface related activities of tissue factor/VIIa, antithrombin, TFPI, and plasminogen will be measured. If the endothelium-targeted transgenic mice produced in projects #1 and 4 show evidence of thrombosis, similar studies will be carried out with the corresponding tissue cell cultures. 3) Screening for abnormalities of liver function and other metabolic derangements. A panel of chemistry tests will be established to evaluate the overall function of the liver and the metabolic status of control and transgenic mice. When abnormal test results are found in one of these screening tests, further studies will be performed as needed, in close co- operation with the individual project leaders.

小鼠血液学/凝血核心的主要功能是 检查血液学和相关的代谢异常， 不同项目生产的不同小鼠品系。的 该核心将执行以下任务：1）识别 血细胞数量和形态异常，以及 止血效果。测量将包括：a）血液学 参数：红细胞、白色细胞的定量 （包括子集）和全血中的血小板。B）止血 参数：出血时间、凝血酶原时间（PT）、活化部分 凝血活酶时间（APTT）和凝血酶时间。c）纤溶酶 参数：稀释全血凝块溶解时间。d）抗凝 参数：蛋白C、蛋白S和抗凝血酶活性。（二） 识别内皮细胞功能异常， 止血基因改变的内皮细胞 表面糖胺聚糖（项目#5）在血液凝固和 将通过使用内皮细胞培养物评估纤维蛋白溶解 来源于对照和转基因小鼠的不同组织。细胞 组织因子/VIIa，抗凝血酶， 将测量TFPI和纤溶酶原。如果内皮靶向 在项目#1和4中生产的转基因小鼠显示出以下证据： 血栓形成，类似的研究将进行与 相应的组织细胞培养物。3)异常筛查 肝功能和其他代谢紊乱一组 将建立化学测试来评估整体功能 以及对照组和转基因组的代谢状态 小鼠当在这些筛查中发现异常检测结果时， 测试，进一步的研究将根据需要进行，在密切合作， 与个别项目负责人合作。