Core--Hematology /coagulation

核心--血液学/凝血

• 批准号: 7510388
• 负责人: DZUNG LE
• 金额: $ 17.09万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7510388
• 关键词: Address; Affect; Antibodies; Biological Assay; Biological Process; Biology; Blood Coagulation Factor; Coagulation Process; Defect; Development; Disease; Enzymes; Exhibits; Future; Glycoproteins; Hematology; Hematopoiesis; Hematopoietic; Hemostatic Agents; Hemostatic function; Hormones; Human; Individual; Metabolic; Mouse Strains; Oligosaccharides; Personal Satisfaction; Play; Process; Program Research Project Grants; Role; Screening procedure; Testing; cell type; design; glycosylation; glycosyltransferase; research study; secretory protein

DESCRIPTION (provided by applicant): Overview: The importance of glycosylation in the biology of lympho-hematopoietic development and function is well established. In human, several disorders of hemostasis have been associated with abnormal oligosaccharide processing (1-8). Most secretory proteins (coagulation factors, hormones, enzymes, and antibodies) are glycoproteins. Previous studies have shown that glycosylation plays an important role in their biological function and in turnover, which in turn may play a role in governing the metabolic state of an individual. In recent years, genetically altered mouse strains that lack specific glycosyltransferases have been found to exhibit significant abnormalities in their hematopoietic, hemostatic and metabolic functions (9-14). Some of these abnormalities have been subtle and would have been missed in absence of standardized panels of screening tests designed to detect these abnormalities as well as assays developed to further evaluate possible specific defects. The functions of the Hematology/Coagulation Core are to perform screening assays to identify phenotypic abnormalities in hematopoiesis, hemostasis, and metabolic functions of the genetically altered mouse strains to be generated by the four projects of this Program Project Grant (PPG). Results of the studies by this core will be recorded, analyzed, interpreted and made available to the corresponding project leaders in a form that is readily accessed and compared, such as Excel spreadsheets and common graphic files. The phenotypic results obtained will help indicate specific cell types affected and possible future experiments by the projects in further addressing the specific aims of this PPG.

描述（由申请人提供）： 概述：糖基化在淋巴造血生物学中的重要性 的发展和功能已经完善。在人类中，有几种止血障碍 与异常寡糖加工有关 (1-8)。大多数分泌蛋白 （凝血因子、激素、酶和抗体）是糖蛋白。之前的研究 已经表明糖基化在其生物学功能和 周转率，这反过来又可能在控制个体的代谢状态中发挥作用。在 近年来，缺乏特定糖基转移酶的基因改造小鼠品系已 被发现在造血、止血和 代谢功能 (9-14)。其中一些异常是微妙的，可能会 由于缺乏旨在检测这些的标准化筛选测试组而被错过 异常情况以及为进一步评估可能的特定缺陷而开发的检测方法。这 血液学/凝血核心的功能是进行筛选分析以识别 造血、止血和代谢功能的表型异常 该计划项目的四个项目将产生转基因小鼠品系 格兰特（PPG）。该核心的研究结果将被记录、分析、解释和 以易于访问和使用的形式提供给相应的项目负责人 相比之下，例如 Excel 电子表格和常见的图形文件。表型结果 获得的结果将有助于表明受影响的特定细胞类型以及未来可能进行的实验 进一步实现本 PPG 特定目标的项目。